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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-30400 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAM68 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurakt, PC-12, Rat2.
- Concentration
- 0.92 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SAM68 using 50 µg mouse brain extract. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SAM68 polyclonal antibody (Product # PA5-30400) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SAM68 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a SAM68 Polyclonal Antibody (Product # PA5-30400) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SAM68 Polyclonal Antibody (Product # PA5-30400). Sample (30 µg of whole cell lysate). Lane A: Jurkat. 10% SDS PAGE. SAM68 Polyclonal Antibody (Product # PA5-30400) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SAM68 Polyclonal Antibody detects SAM68 protein by western blot analysis. A. 30 µg PC-12 whole cell extract. B. 30 µg Rat2 whole cell extract.7.5 % SDS-PAGE. SAM68 Polyclonal Antibody (Product # PA5-30400) dilution: 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SAM68 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: SAM68 Polyclonal Antibody (Product # PA5-30400) diluted at 1:2000. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of SAM68 was performed in paraffin-embedded mouse brain tissue using SAM68 Polyclonal Antibody (Product # PA5-30400) at a dilution of 1:500. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SAM68 Polyclonal Antibody detects SAM68 protein at cytosol on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SAM68 Polyclonal Antibody (Product # PA5-30400) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
