PA5-46966

antibody from Invitrogen Antibodies

Targeting: TNXB TNXB1, TNXB2, TNXBS, XB, XBS

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46966

Provider

Invitrogen Antibodies

Product name

Tenascin X Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This antibody detects recombinant human Tenascin X isoform 2 in direct ELISAs and human Tenascin X in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Tenascin R is observed. Reconstitute in sterile PBS to a final concentration of 0.2 mg/mL.

Reactivity

Human

Host

Sheep

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-human Tenascin X Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46966) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Tenascin X at approximately 470 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Tenascin X in human heart tissue. Samples were incubated in Tenascin X polyclonal antibody (Product # PA5-46966) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Tenascin X at approximately 470 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of TNXB in 0.2 mg/mL lysates of human heart tissue. Samples were incubated in TNXB polyclonal antibody (Product # PA5-46966) using a dilution of 50 µg/mL followed by HRP-conjugated Anti-Sheep IgG at a dilution of 1:50. A specific band was detected for Tenascin X at approximately 400 kDa (as indicated). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Tenascin X in immersion fixed paraffin-embedded sections of human lung mesothelioma. Samples were incubated in Tenascin X polyclonal antibody (Product # PA5-46966) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to stromal cells.