H00007148-M01

antibody from Abnova Corporation

Targeting: TNXB TNXB1, TNXB2, TNXBS, XB, XBS

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007148-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007148-M01, RRID:AB_425721

Product name

TNXB monoclonal antibody (M01), clone 2D3-1B10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant TNXB.

Antigen sequence

MRLSWSVAQGPFDSFVVQYEDTNGQPQALLVDGDQ
SKILISGLEPSTPYRFLLYGLHEGKRLGPLSAEGT
TGLAPAGQTSEESRPRLSQLSVTDVTTSSLRLNWE
APPGAFDSFLLRFGVPSPSTLEPHPRPLLQRELMV
PGTRHSAVLRDLRSGTLYSLTLYGLRGPHKADSIQ
GTARTLSPVLESPRDLQFSEIRETSAKVNWMPPPS
RADSFKVSYLLADGGEPQSVQVDGQARTQKLQGLI
PGARYEVTVVSVRGFEESEPLTGFLTTVPDGPTQL
RALNLTEGFAVLHWKPPQNPVDTYDVQVTAPGAPP
LQAETPGSAVDYPLHDLVLHTNYTATVRGLRGPNL
TSPASITFTTGLEAPRDLEAKEVTPRTALLTWTEP
PVRPAGYLLSFHTPGGQNQEILLPGGITSHQLLGL
FPSTSYNARLQAMWGQSLLPPVSTSFTTGGLRIPF
PRDCGEEMQNGAGASRTSTIFLNGNRERPLNVFCD
METDGGGWLVFQRRMDGQTDFWRDWEDYAHGFGNI
SGEFWLGNEALHSLTQAGDYSMRVDLRAGDEAVFA
QYDSFHVDSAAEYYRLHLEGYHGTAGDSMSYHSGS
VFSARDRDPNSLLISCAVSYRGAWWYRNCHYANLN
GLYGSTVDHQGVSWYHWKGFEFSVPFTEMKLRPRN
FRSPAGGG


Isotype

IgG

Antibody clone number

2D3-1B10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of TNXB expression in transfected 293T cell line by TNXB monoclonal antibody (M01), clone 2D3-1B10.Lane 1: TNXB transfected lysate (Predicted MW: 74 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged TNXB is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of TNXB transfected lysate using anti-TNXB monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TNXB MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol