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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF6034 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Nogo-B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse Nogo-B in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Nogo-A and recombinant rat Nogo-A is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Nogo-B by Western Blot. Western blot shows lysates of TK-6 human lymphoblast cell line, Neuro-2A mouse neuroblastoma cell line, and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of Human/Mouse Nogo-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6034) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Nogo-B at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Nogo-B in Human Heart. Nogo-B was detected in immersion fixed paraffin-embedded sections of human heart using Human/Mouse Nogo-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6034) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
