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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- MAB6596 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Nogo-B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Nogo-B in direct ELISAs. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) Nogo-B and recombinant rat Nogo-A and no cross-reactivity with rhNogo-A is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q99P72- Isotype
- IgG
- Antibody clone number
- 523808
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Nogo-B in Mouse Heart. Nogo-B was detected in immersion fixed frozen sections of adult mouse heart using Mouse Nogo-B Monoclonal Antibody (Catalog # MAB6596) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cardiac outflow tract. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
