PA5-47773

antibody from Invitrogen Antibodies

Targeting: RTN4 ASY, KIAA0886, NOGO, NSP-CL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47773

Provider

Invitrogen Antibodies

Product name

Nogo-C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Nogo-A and rhNogo-B is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of human skeletal muscle. PVDF Membrane was probed with 1 µg/mL of human Nogo-C Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47773) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Nogo-C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nogo-C in human skeletal muscle. Samples were incubated in Nogo-C polyclonal antibody (Product # PA5-47773) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Nogo‚C at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Nogo-C in immersion fixed paraffin-embedded sections of human brain (medulla). Samples were incubated in Nogo-C polyclonal antibody (Product # PA5-47773) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.