PA5-47807

antibody from Invitrogen Antibodies

Targeting: RTN4 ASY, KIAA0886, NOGO, NSP-CL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47807

Provider

Invitrogen Antibodies

Product name

Nogo-B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs, less than 1% cross-reactivity with recombinant human Nogo-A and recombinant rat Nogo-A is observed.

Concentration

0.2 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysates of TK-6 human lymphoblast cell line, Neuro-2A mouse neuroblastoma cell line, and C2C12 mouse myoblast cell line. PVDF Membrane was probed with 1 µg/mL of human/mouse Nogo-B Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47807) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Nogo-B at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Nogo-B in TK‚6 human lymphoblast cell line, Neuro‚2A mouse neuroblastoma cell line, and C2C12 mouse myoblast cell line. Samples were incubated in Nogo-B polyclonal antibody (Product # PA5-47807) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Nogo‚B at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Nogo-B in immersion fixed paraffin-embedded sections of human heart. Samples were incubated in Nogo-B polyclonal antibody (Product # PA5-47807) using a dilution of 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells.