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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- A01844-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Nogo A/RTN4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Nogo A/RTN4 detection. Tested with WB, IHC-P, ICC/IF, IF, FCM in Human.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Icariside II affects hippocampal neuron axon regeneration and improves learning and memory in a chronic cerebral hypoperfusion rat model.
Methylprednisolone inhibits Nogo-A protein expression after acute spinal cord injury.
Expression of Nogo-A on the retina in rat model with chronic ocular hypertension.
Liu T, He F, Yan J, Kuang W, Yu C
International journal of clinical and experimental pathology 2019;12(3):826-834
International journal of clinical and experimental pathology 2019;12(3):826-834
Methylprednisolone inhibits Nogo-A protein expression after acute spinal cord injury.
Fu Z, Lu H, Jiang J, Jiang H, Zhang Z
Neural regeneration research 2013 Feb 15;8(5):404-9
Neural regeneration research 2013 Feb 15;8(5):404-9
Expression of Nogo-A on the retina in rat model with chronic ocular hypertension.
Nie QZ, Liu ZL, Sha Q, Gao DW
International journal of ophthalmology 2010;3(2):112-3
International journal of ophthalmology 2010;3(2):112-3
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of Nogo A/RTN4 using anti-Nogo A/RTN4 antibody (A01844-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nogo A/RTN4 antigen affinity purified polyclonal antibody (Catalog # A01844-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nogo A/RTN4 at approximately 180KD. The expected band size for Nogo A/RTN4 is at 180KD.
- Additional image
