55114-1-AP

antibody from Proteintech Group

Targeting: NOTCH3 CADASIL, CASIL

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

55114-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#55114-1-AP, RRID:AB_10858393

Product name

NOTCH3 antibody

Antibody type

Polyclonal

Description

NOTCH3 antibody (Cat. #55114-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Huaier suppresses cell viability, migration and invasion in human non-small cell lung cancer via lncRNA DLEU2/miR-212-5p/ELF3 axis.

Wu T, Liu S, Chen W, Zhao D, Lu Z
International journal of medical sciences 2024;21(2):319-331

---

Anoikis patterns via machine learning strategy and experimental verification exhibit distinct prognostic and immune landscapes in melanoma.

Liu J, Ma R, Chen S, Lai Y, Liu G
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2024 May;26(5):1170-1186

---

Integrated analysis reveals the dysfunction of intercellular communication and metabolic signals in dilated cardiomyopathy.

Shi R, Ma X, Zhou M, Xie X, Xu L
Heliyon 2024 Mar 15;10(5):e26803

---

FPR3 reprograms glycolytic metabolism and stemness in gastric cancer via calcium-NFATc1 pathway.

Wang L, Mao X, Yu X, Su J, Li Z, Chen Z, Ren Y, Huang H, Wang W, Zhao C, Hu Y
Cancer letters 2024 Jul 1;593:216841

---

Molecular mechanism underlying miR-204-5p regulation of adipose-derived stem cells differentiation into cells from three germ layers.

Wang Z, Bi M, Zhe X, Wang X, Dai B, Han X, Ren B, Liang H, Liu D
Cell death discovery 2024 Feb 22;10(1):95

---

GHITM regulates malignant phenotype and sensitivity to PD-1 blockade of renal cancer cells via Notch signalling.

Huang S, Liu J, Hu J, Hou Y, Hu M, Zhang B, Luo H, Fu S, Chen Y, Liu X, Chen Z, Wang L
Journal of cellular and molecular medicine 2024 Apr;28(8):e18290

---

SNORA73B promotes endometrial cancer progression through targeting MIB1 and regulating host gene RCC1 alternative splicing.

Chen X, Li QH, Xie BM, Ji YM, Han Y, Zhao Y
Journal of cellular and molecular medicine 2023 Oct;27(19):2890-2905

---

LncRNA-Gm9866 promotes liver fibrosis by activating TGFβ/Smad signaling via targeting Fam98b.

Liao X, Ruan X, Yao P, Yang D, Wu X, Zhou X, Jing J, Wei D, Liang Y, Zhang T, Qin S, Jiang H
Journal of translational medicine 2023 Nov 2;21(1):778

---

Lysine 2-hydroxyisobutyrylation of NAT10 promotes cancer metastasis in an ac4C-dependent manner.

Liao L, He Y, Li SJ, Yu XM, Liu ZC, Liang YY, Yang H, Yang J, Zhang GG, Deng CM, Wei X, Zhu YD, Xu TY, Zheng CC, Cheng C, Li A, Li ZG, Liu JB, Li B
Cell research 2023 May;33(5):355-371

---

Di-(2-ethylhexyl) phthalate (DEHP) promoted hepatic lipid accumulation by activating Notch signaling pathway.

Zhang Y, Qian H, Wang J, Zhu Y, Miao X, Li X, Yin J, Zhang R, Ye J, Huo C, Zhao W, Ye L
Environmental toxicology 2023 Jul;38(7):1628-1640

---

Expressions of EZH2 and NOTCH3 pathway in osteosarcoma and their roles in osteosarcoma stem cells.

Yan G, Lv Y, Lai X, Li Z, Ye D, Wang W, Qian Y, Yu H, Wang Z
Polish journal of pathology : official journal of the Polish Society of Pathologists 2022;73(4):343-351

---

Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer.

Zhou Y, Xiao L, Long G, Cao J, Liu S, Tao Y, Zhou L, Tang J
Frontiers in immunology 2022;13:921182

---

hsa_circ_0001741 promotes esophageal squamous cell carcinoma stemness, invasion and migration by sponging miR-491-5p to upregulate NOTCH3 expression.

Li L, Lei K, Lyu Y, Tan B, Liang R, Wu D, Wang K, Wang W, Lin H, Wang M
American journal of cancer research 2022;12(5):2012-2031

---

A comprehensive role evaluation and mechanism exploration of POGLUT2 in pan-cancer.

Xu X, Xie G, Xie M, Liu Q
Frontiers in oncology 2022;12:962540

---

A tumor-suppressive function for Notch3 in the parous mammary gland.

Chung WC, Egan SE, Xu K
Development (Cambridge, England) 2022 Oct 1;149(19)

---

Evaluation of NOTCH family genes' expression and prognostic value in prostate cancer.

Qing Y, Wang Y, Hu C, Zhang H, Huang Y, Zhang Z, Ma T, Zhang S, Li K
Translational andrology and urology 2022 May;11(5):627-642

---

C/EBPβ mediates anti-proliferative effects of 1,25(OH)2D on differentiated thyroid carcinoma cells.

Xu F, Ling Y, Yuan J, Zeng Q, Li L, Dai D, Xia X, Sun R, Zhang R, Xie Z
Endocrine-related cancer 2022 May 9;29(6):321-334

---

JAGGED-NOTCH3 signaling in vascular remodeling in pulmonary arterial hypertension.

Zhang Y, Hernandez M, Gower J, Winicki N, Morataya X, Alvarez S, Yuan JX, Shyy J, Thistlethwaite PA
Science translational medicine 2022 May 4;14(643):eabl5471

---

Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma.

Huang X, Jiang L, Lu S, Yuan M, Lin H, Li B, Wen Z, Zhong Y
Oncology reports 2022 Jul;48(1)

---

Role of notch signaling pathway in Muc5ac secretion induced by atmospheric PM(2.5) in rats.

Liu Y, Zhou L, Wu H, Wang Y, Danzengluobu, Zhang B
Ecotoxicology and environmental safety 2022 Jan 1;229:113052

---

Piezo1-mediated fluid shear stress promotes OPG and inhibits RANKL via NOTCH3 in MLO-Y4 osteocytes.

Liu Z, Tang Y, He L, Geng B, Lu F, He J, Yi Q, Liu X, Zhang K, Wang L, Xia Y, Jiang J
Channels (Austin, Tex.) 2022 Dec;16(1):127-136

---

Akt1-dependent expression of angiopoietin 1 and 2 in vascular smooth muscle cells leads to vascular stabilization.

Ha JM, Jin SY, Lee HS, Kum HJ, Vafaeinik F, Ha HK, Song SH, Kim CD, Bae SS
Experimental & molecular medicine 2022 Aug;54(8):1133-1145

---

METTL3-mediated m(6)A modification of TIMP2 mRNA promotes podocyte injury in diabetic nephropathy.

Jiang L, Liu X, Hu X, Gao L, Zeng H, Wang X, Huang Y, Zhu W, Wang J, Wen J, Meng X, Wu Y
Molecular therapy : the journal of the American Society of Gene Therapy 2022 Apr 6;30(4):1721-1740

---

UBE3A activates the NOTCH pathway and promotes esophageal cancer progression by degradation of ZNF185.

Zheng Z, Zhang B, Yu H, Li S, Song N, Jin X, Li J
International journal of biological sciences 2021;17(12):3024-3035

---

Elabela gene therapy promotes angiogenesis after myocardial infarction.

Jin L, Pan Y, Li Q, Li J, Wang Z
Journal of cellular and molecular medicine 2021 Sep;25(17):8537-8545

---

GALNT2 promotes cell proliferation, migration, and invasion by activating the Notch/Hes1-PTEN-PI3K/Akt signaling pathway in lung adenocarcinoma.

Wang W, Sun R, Zeng L, Chen Y, Zhang N, Cao S, Deng S, Meng X, Yang S
Life sciences 2021 Jul 1;276:119439

---

Selective Targeting of Class I Histone Deacetylases in a Model of Human Osteosarcoma.

Torres HM, VanCleave AM, Vollmer M, Callahan DL, Smithback A, Conn JM, Rodezno-Antunes T, Gao Z, Cao Y, Afeworki Y, Tao J
Cancers 2021 Aug 20;13(16)

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Notch1 haploinsufficiency in mice accelerates adipogenesis.

Yamaguchi K, Hayashi M, Uchida Y, Cheng XW, Nakayama T, Matsushita T, Murohara T, Takeshita K
Scientific reports 2021 Aug 18;11(1):16761

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Notch3 Modulates Cardiac Fibroblast Proliferation, Apoptosis, and Fibroblast to Myofibroblast Transition via Negative Regulation of the RhoA/ROCK/Hif1α Axis.

Shi J, Xiao P, Liu X, Chen Y, Xu Y, Fan J, Yin Y
Frontiers in physiology 2020;11:669

---

The effect of Notch signal pathway on PM(2.5)-induced Muc5ac in Beas-2B cells.

Liu Y, Zhou T, Sun L, Wang H, Zhou L
Ecotoxicology and environmental safety 2020 Oct 15;203:110956

---

Enhanced Notch3 signaling contributes to pulmonary emphysema in a Murine Model of Marfan syndrome.

Jespersen K, Liu Z, Li C, Harding P, Sestak K, Batra R, Stephenson CA, Foley RT, Greene H, Meisinger T, Baxter BT, Xiong W
Scientific reports 2020 Jul 2;10(1):10949

---

Notch3 promotes prostate cancer-induced bone lesion development via MMP-3.

Ganguly SS, Hostetter G, Tang L, Frank SB, Saboda K, Mehra R, Wang L, Li X, Keller ET, Miranti CK
Oncogene 2020 Jan;39(1):204-218

---

Overexpression of AMPD2 indicates poor prognosis in colorectal cancer patients via the Notch3 signaling pathway.

Gao QZ, Qin Y, Wang WJ, Fei BJ, Han WF, Jin JQ, Gao X
World journal of clinical cases 2020 Aug 6;8(15):3197-3208

---

Circ_PUM1 promotes the development of endometrial cancer by targeting the miR-136/NOTCH3 pathway.

Zong ZH, Liu Y, Chen S, Zhao Y
Journal of cellular and molecular medicine 2020 Apr;24(7):4127-4135

---

Upregulated MMP28 in Hepatocellular Carcinoma Promotes Metastasis via Notch3 Signaling and Predicts Unfavorable Prognosis.

Zhou J, Zheng X, Feng M, Mo Z, Shan Y, Wang Y, Jin J
International journal of biological sciences 2019;15(4):812-825

---

Exosomal Notch3 from high glucose-stimulated endothelial cells regulates vascular smooth muscle cells calcification/aging.

Lin X, Li S, Wang YJ, Wang Y, Zhong JY, He JY, Cui XJ, Zhan JK, Liu YS
Life sciences 2019 Sep 1;232:116582

---

DLL3 regulates the migration and invasion of small cell lung cancer by modulating Snail.

Furuta M, Kikuchi H, Shoji T, Takashima Y, Kikuchi E, Kikuchi J, Kinoshita I, Dosaka-Akita H, Sakakibara-Konishi J
Cancer science 2019 May;110(5):1599-1608

---

Downregulation of Notch Signaling in Kras-Induced Gastric Metaplasia.

Chung WC, Zhou Y, Atfi A, Xu K
Neoplasia (New York, N.Y.) 2019 Aug;21(8):810-821

---

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Zhong X, Cui P, Cai Y, Wang L, He X, Long P, Lu K, Yan R, Zhang Y, Pan X, Zhao X, Li W, Zhang H, Zhou Q, Gao P
Cell metabolism 2019 Apr 2;29(4):979-992.e4

---

Laminin-521 promotes quiescence in isolated stellate cells from rat liver.

Rohn F, Kordes C, Castoldi M, Götze S, Poschmann G, Stühler K, Herebian D, Benk AS, Geiger F, Zhang T, Spatz JP, Häussinger D
Biomaterials 2018 Oct;180:36-51

---

Mild hypothermia inhibits the Notch 3 and Notch 4 activation and seizure after stroke in the rat model.

Yang GS, Zhou XY, An XF, Liu XJ, Zhang YJ, Yu D
Pathology, research and practice 2018 Jul;214(7):1008-1016

---

Notch signaling via regulation of RB and p-AKT but not PIK3CG contributes to MIA PaCa-2 cell growth and migration to affect pancreatic carcinogenesis.

Zhang S, Liu J, Xu K, Li Z
Oncology letters 2018 Feb;15(2):2105-2110

---

Kras(G12D) upregulates Notch signaling to induce gallbladder tumorigenesis in mice.

Chung WC, Wang J, Zhou Y, Xu K
Oncoscience 2017 Sep;4(9-10):131-138

---

Lunatic Fringe and p53 Cooperatively Suppress Mesenchymal Stem-Like Breast Cancer.

Chung WC, Zhang S, Challagundla L, Zhou Y, Xu K
Neoplasia (New York, N.Y.) 2017 Nov;19(11):885-895

---

Combined Methylome and Transcriptome Analysis During Rat Hepatic Stellate Cell Activation.

Schumacher EC, Götze S, Kordes C, Benes V, Häussinger D
Stem cells and development 2017 Dec 15;26(24):1759-1770

---

Lunatic Fringe is a potent tumor suppressor in Kras-initiated pancreatic cancer.

Zhang S, Chung WC, Xu K
Oncogene 2016 May 12;35(19):2485-95

---

Manic fringe promotes a claudin-low breast cancer phenotype through notch-mediated PIK3CG induction.

Zhang S, Chung WC, Wu G, Egan SE, Miele L, Xu K
Cancer research 2015 May 15;75(10):1936-43

---

Neuropathological and biochemical assessments of an Alzheimer's disease patient treated with the γ-secretase inhibitor semagacestat.

Roher AE, Maarouf CL, Kokjohn TA, Whiteside CM, Kalback WM, Serrano G, Belden C, Liebsack C, Jacobson SA, Sabbagh MN, Beach TG
American journal of neurodegenerative disease 2014;3(3):115-33

---

Targeting Met and Notch in the Lfng-deficient, Met-amplified triple-negative breast cancer.

Zhang S, Chung WC, Miele L, Xu K
Cancer biology & therapy 2014 May;15(5):633-42

---

Tumor-suppressive activity of Lunatic Fringe in prostate through differential modulation of Notch receptor activation.

Zhang S, Chung WC, Wu G, Egan SE, Xu K
Neoplasia (New York, N.Y.) 2014 Feb;16(2):158-67

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 55114-1-AP(NOTCH3 antibody) at dilution of 1:500

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 55114-1-AP(NOTCH3 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The NOTCH3 antibody from Proteintech is a rabbit polyclonal antibody to a peptide of human NOTCH3. This antibody recognizes human, mouse, rat antigen. The NOTCH3 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

55114-1-AP.pdf

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