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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-2215 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2215, RRID:AB_10002406
- Product name
- Rabbit Polyclonal SREBP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Astroglial biotin deprivation under endoplasmic reticulum stress uncouples BCAA-mTORC1 role in lipid synthesis to prolong autophagy inhibition in the aging brain.
The Absence of HIF-1α Increases Susceptibility to Leishmania donovani Infection via Activation of BNIP3/mTOR/SREBP-1c Axis.
The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
Non-alcoholic Fatty Liver Disease Induced by Perinatal Exposure to Bisphenol a Is Associated With Activated mTOR and TLR4/NF-κB Signaling Pathways in Offspring Rats.
Heat Shock Factor 1 Is a Direct Antagonist of AMP-Activated Protein Kinase.
Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly.
Low abundance of mitofusin 2 in dairy cows with moderate fatty liver is associated with alterations in hepatic lipid metabolism.
Soy Isoflavones Ameliorate Fatty Acid Metabolism of Visceral Adipose Tissue by Increasing the AMPK Activity in Male Rats with Diet-Induced Obesity (DIO).
Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway.
Adaptations of hepatic lipid metabolism and mitochondria in dairy cows with mild fatty liver.
Deficiency of cholesterol 7α-hydroxylase in bile acid synthesis exacerbates alcohol-induced liver injury in mice.
PTEN Down-Regulation Promotes β-Oxidation to Fuel Hypertrophic Liver Growth After Hepatectomy in Mice.
Red algae (Gelidium amansii) hot-water extract ameliorates lipid metabolism in hamsters fed a high-fat diet.
Upregulation of miR-181a impairs hepatic glucose and lipid homeostasis.
BHBA influences bovine hepatic lipid metabolism via AMPK signaling pathway.
The macrophage A2B adenosine receptor regulates tissue insulin sensitivity.
Marine- and plant-derived ω-3 fatty acids differentially regulate prostate cancer cell proliferation.
The A2b adenosine receptor modulates glucose homeostasis and obesity.
The Niemann-Pick C1 gene is downregulated in livers of C57BL/6J mice by dietary fatty acids, but not dietary cholesterol, through feedback inhibition of the SREBP pathway.
Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice.
Activated sterol regulatory element-binding protein-2 suppresses hepatocyte nuclear factor-4-mediated Cyp3a11 expression in mouse liver.
Species-specific dibutyl phthalate fetal testis endocrine disruption correlates with inhibition of SREBP2-dependent gene expression pathways.
Ganesan D, Ramaian Santhaseela A, Rajasekaran S, Selvam S, Jayavelu T
Journal of neurochemistry 2020 Sep;154(5):562-575
Journal of neurochemistry 2020 Sep;154(5):562-575
The Absence of HIF-1α Increases Susceptibility to Leishmania donovani Infection via Activation of BNIP3/mTOR/SREBP-1c Axis.
Mesquita I, Ferreira C, Moreira D, Kluck GEG, Barbosa AM, Torrado E, Dinis-Oliveira RJ, Gonçalves LG, Beauparlant CJ, Droit A, Berod L, Sparwasser T, Bodhale N, Saha B, Rodrigues F, Cunha C, Carvalho A, Castro AG, Estaquier J, Silvestre R
Cell reports 2020 Mar 24;30(12):4052-4064.e7
Cell reports 2020 Mar 24;30(12):4052-4064.e7
The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
Xu D, Wang Z, Xia Y, Shao F, Xia W, Wei Y, Li X, Qian X, Lee JH, Du L, Zheng Y, Lv G, Leu JS, Wang H, Xing D, Liang T, Hung MC, Lu Z
Nature 2020 Apr;580(7804):530-535
Nature 2020 Apr;580(7804):530-535
Non-alcoholic Fatty Liver Disease Induced by Perinatal Exposure to Bisphenol a Is Associated With Activated mTOR and TLR4/NF-κB Signaling Pathways in Offspring Rats.
Lin R, Wu D, Wu FJ, Meng Y, Zhang JH, Wang XG, Jia LH
Frontiers in endocrinology 2019;10:620
Frontiers in endocrinology 2019;10:620
Heat Shock Factor 1 Is a Direct Antagonist of AMP-Activated Protein Kinase.
Su KH, Dai S, Tang Z, Xu M, Dai C
Molecular cell 2019 Nov 21;76(4):546-561.e8
Molecular cell 2019 Nov 21;76(4):546-561.e8
Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly.
Jia H, Li X, Liu G, Loor JJ, Bucktrout R, Sun X, Li G, Shu X, Dong J, Wang Y, Zuo R, Wang Z, Li X
Journal of dairy science 2019 Jan;102(1):833-845
Journal of dairy science 2019 Jan;102(1):833-845
Low abundance of mitofusin 2 in dairy cows with moderate fatty liver is associated with alterations in hepatic lipid metabolism.
Dong J, Loor JJ, Zuo R, Chen X, Liang Y, Wang Y, Shu X, Sun X, Jia H, Liu G, Wang Z, Li X, Li X
Journal of dairy science 2019 Aug;102(8):7536-7547
Journal of dairy science 2019 Aug;102(8):7536-7547
Soy Isoflavones Ameliorate Fatty Acid Metabolism of Visceral Adipose Tissue by Increasing the AMPK Activity in Male Rats with Diet-Induced Obesity (DIO).
Tan J, Huang C, Luo Q, Liu W, Cheng D, Li Y, Xia Y, Li C, Tang L, Fang J, Pan K, Ou Y, Cheng A, Chen Z
Molecules (Basel, Switzerland) 2019 Aug 1;24(15)
Molecules (Basel, Switzerland) 2019 Aug 1;24(15)
Acetic Acid Influences BRL-3A Cell Lipid Metabolism via the AMPK Signalling Pathway.
Li L, He M, Xiao H, Liu X, Wang K, Zhang Y
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(5):2021-2030
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;45(5):2021-2030
Adaptations of hepatic lipid metabolism and mitochondria in dairy cows with mild fatty liver.
Du X, Shen T, Wang H, Qin X, Xing D, Ye Q, Shi Z, Fang Z, Zhu Y, Yang Y, Peng Z, Zhao C, Lv B, Li X, Liu G, Li X
Journal of dairy science 2018 Oct;101(10):9544-9558
Journal of dairy science 2018 Oct;101(10):9544-9558
Deficiency of cholesterol 7α-hydroxylase in bile acid synthesis exacerbates alcohol-induced liver injury in mice.
Donepudi AC, Ferrell JM, Boehme S, Choi HS, Chiang JYL
Hepatology communications 2018 Jan;2(1):99-112
Hepatology communications 2018 Jan;2(1):99-112
PTEN Down-Regulation Promotes β-Oxidation to Fuel Hypertrophic Liver Growth After Hepatectomy in Mice.
Kachaylo E, Tschuor C, Calo N, Borgeaud N, Ungethüm U, Limani P, Piguet AC, Dufour JF, Foti M, Graf R, Clavien PA, Humar B
Hepatology (Baltimore, Md.) 2017 Sep;66(3):908-921
Hepatology (Baltimore, Md.) 2017 Sep;66(3):908-921
Red algae (Gelidium amansii) hot-water extract ameliorates lipid metabolism in hamsters fed a high-fat diet.
Yang TH, Yao HT, Chiang MT
Journal of food and drug analysis 2017 Oct;25(4):931-938
Journal of food and drug analysis 2017 Oct;25(4):931-938
Upregulation of miR-181a impairs hepatic glucose and lipid homeostasis.
Du X, Yang Y, Xu C, Peng Z, Zhang M, Lei L, Gao W, Dong Y, Shi Z, Sun X, Wang Z, Li X, Li X, Liu G
Oncotarget 2017 Oct 31;8(53):91362-91378
Oncotarget 2017 Oct 31;8(53):91362-91378
BHBA influences bovine hepatic lipid metabolism via AMPK signaling pathway.
Deng Q, Liu G, Liu L, Zhang Y, Yin L, Shi X, Wang J, Yuan X, Sun G, Li Y, Yang W, Guo L, Zhang R, Wang Z, Li X, Li X
Journal of cellular biochemistry 2015 Jun;116(6):1070-9
Journal of cellular biochemistry 2015 Jun;116(6):1070-9
The macrophage A2B adenosine receptor regulates tissue insulin sensitivity.
Johnston-Cox H, Eisenstein AS, Koupenova M, Carroll S, Ravid K
PloS one 2014;9(6):e98775
PloS one 2014;9(6):e98775
Marine- and plant-derived ω-3 fatty acids differentially regulate prostate cancer cell proliferation.
Eser PO, Vanden Heuvel JP, Araujo J, Thompson JT
Molecular and clinical oncology 2013 May;1(3):444-452
Molecular and clinical oncology 2013 May;1(3):444-452
The A2b adenosine receptor modulates glucose homeostasis and obesity.
Johnston-Cox H, Koupenova M, Yang D, Corkey B, Gokce N, Farb MG, LeBrasseur N, Ravid K
PloS one 2012;7(7):e40584
PloS one 2012;7(7):e40584
The Niemann-Pick C1 gene is downregulated in livers of C57BL/6J mice by dietary fatty acids, but not dietary cholesterol, through feedback inhibition of the SREBP pathway.
Jelinek D, Castillo JJ, Richardson LM, Luo L, Heidenreich RA, Garver WS
The Journal of nutrition 2012 Nov;142(11):1935-42
The Journal of nutrition 2012 Nov;142(11):1935-42
Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice.
Yang A, Gyulay G, Mitchell M, White E, Trigatti BL, Igdoura SA
Journal of lipid research 2012 Dec;53(12):2573-85
Journal of lipid research 2012 Dec;53(12):2573-85
Activated sterol regulatory element-binding protein-2 suppresses hepatocyte nuclear factor-4-mediated Cyp3a11 expression in mouse liver.
Inoue S, Yoshinari K, Sugawara M, Yamazoe Y
Molecular pharmacology 2011 Jan;79(1):148-56
Molecular pharmacology 2011 Jan;79(1):148-56
Species-specific dibutyl phthalate fetal testis endocrine disruption correlates with inhibition of SREBP2-dependent gene expression pathways.
Johnson KJ, McDowell EN, Viereck MP, Xia JQ
Toxicological sciences : an official journal of the Society of Toxicology 2011 Apr;120(2):460-74
Toxicological sciences : an official journal of the Society of Toxicology 2011 Apr;120(2):460-74
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SREBP1 Antibody [NB100-2215] - Detection of SREBP1. Lane 1: human liver. Lane 2: mouse liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SREBP1 Antibody [NB100-2215] - Image shows a specific band for SREBP1 in 1 mg/mL of HeLa cell lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SREBP1 Antibody [NB100-2215] - nalysis of FFPE tissue section of mouse liver with SREBP1 antibody at 1:200. The antibody generated an expected cytoplasmic-nuclear immunostaining of SREBP1 protein in the hepatocytes.
