PA1-337

antibody from Invitrogen Antibodies

Targeting: SREBF1 bHLHd1, SREBP-1c, SREBP1, SREBP1a

Western blot

Antibody data

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Validation data

Product number: PA1-337 - Provider product page
Provider: Invitrogen Antibodies
Product name: SREBP1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA1-337 detects SREBP 1 in mouse and rat samples. PA1-337 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~68 and 120 kDa protein representing SREBP 1 in mouse and rat liver samples as well as rat kidney samples. A predominent band at ~68 kDa (active cleaved site) is seen and a band at ~120 kDa (inactive precursor) may not be seen or it may be diminished. The PA1-337 immunogen is a synthetic peptide corresponding to residues M(32) L Q L I N N Q D S D F P G L F(47) of mouse SREBP 1. This sequence is identical in human and rat. PA1-337 immunizing peptide (Cat. # PEP-274) is available for use in neutralization and control experiments.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

SREBF1 protein structure - PA1-337 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SREBP1 (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP1 polyclonal antibody (Product # PA1-337) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SREBP1 (green) showing staining in the cytoplasm of HepG2 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP1 polyclonal antibody (Product # PA1-337) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of SREBP1 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP1 polyclonal antibody (Product # PA1-337) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. Signal pathway changes and lipid-associated genes and inflammatory cytokine protein changes. (A) Western blotting was performed to reveal the protein expressions of the lipid and inflammatory genes as well as the key genes of the AMPK signal pathway. Lipid synthesis-associated genes and inflammatory genes were inhibited by PSP. (B) P-AMPK and the downstream gene p-ACC was clearly activated by PSP, which demonstrated that the PSP could ameliorate HFD-induced mouse obesity through activating the AMPK signal pathway. (C) Semi-quantification of protein expression levels from part (A) (D) Semi-quantification of protein expression levels from part (B) # P>0.05, *P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5. Effects of GA on macrophage activation, alpha-SMA expression, FAS and SREBP-1c expression in DM-AS rats. (A) Immunohistochemistry was used to detect CD68 and alpha-SMA expression in aortic tissues (magnification, x200). (B) Quantitative analysis of CD68 and alpha-SMA expression from (A). (C) Western blotting detected the expression of FAS and SREBP-1c. ***P

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 5 K17 alters the subcellular localization of SREBP-1 and PPARgamma. (A,B) The protein levels of full-length SPEBP-1 (flSREBP-1) and nuclear SPEBP-1 (nSREBP-1) in HaCaT cells were measured after transfection with K17 siRNA or pEGFP-N1-K17. (C,D) Immunofluorescence analysis of FASN and PPARgamma subcellular localization in HaCaT cells after transfection with K17 siRNA or pEGFP-N1-K17. Scale bars = 10 mum. (E) HaCaT cells were treated with pCMV6-XL5-K17 (K17 LV) and a nuclear fraction was prepared. SREBP-1 and PPARgamma protein was quantitated by Western blot. Lamin A/C and beta-actin were used as loading controls for nuclear and cytosol fractions, respectively. (F) Cells were co-immunoprecipitated with anti-K17 antibody and SREBP-1 and PPARgamma proteins were detected by Western blot. Data are expressed as means +- standard error of the mean from three independent experiments. * p < 0.05.