Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [37]
- Comments [0]
- Validations
- Western blot [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB600-582 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-582, RRID:AB_10001575
- Product name
- Mouse Monoclonal SREBP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian, Hamster, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Antiobesity effect of ethanolic extract of Ramulus mori in differentiated 3T3-L1 adipocytes and high-fat diet-induced obese mice.
Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.
The MYC Oncogene Cooperates with Sterol-Regulated Element-Binding Protein to Regulate Lipogenesis Essential for Neoplastic Growth.
Combined Exposure to Fructose and Bisphenol A Exacerbates Abnormal Lipid Metabolism in Liver of Developmental Male Rats.
Benzyl Isothiocyanate and Phenethyl Isothiocyanate Inhibit Adipogenesis and Hepatosteatosis in Mice with Obesity Induced by a High-Fat Diet.
Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells.
Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis.
Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease.
14-Deoxy-11,12-didehydroandrographolide suppresses adipogenesis of 3 T3-L1 preadipocytes by inhibiting CCAAT/enhancer-binding protein β activation and AMPK-mediated mitotic clonal expansion.
Phytoceuticals in Fenugreek Ameliorate VLDL Overproduction and Insulin Resistance via the Insig Signaling Pathway.
Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation.
Angiotensin II induces cholesterol accumulation and injury in podocytes.
An In Vivo Magnetic Resonance Spectroscopy Study of the Effects of Caloric and Non-Caloric Sweeteners on Liver Lipid Metabolism in Rats.
Telomerase reverse transcriptase (TERT) - enhancer of zeste homolog 2 (EZH2) network regulates lipid metabolism and DNA damage responses in glioblastoma.
Lack of mitochondria-generated acetyl-CoA by pyruvate dehydrogenase complex downregulates gene expression in the hepatic de novo lipogenic pathway.
Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle.
Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling.
MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels.
Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease.
Phytosterols protect against diet-induced hypertriglyceridemia in Syrian golden hamsters.
Alpha-lipoic acid supplementation reduces mTORC1 signaling in skeletal muscle from high fat fed, obese Zucker rats.
Treadmill Exercise Training Modulates Hepatic Cholesterol Metabolism and Circulating PCSK9 Concentration in High-Fat-Fed Mice.
Inhibition of Notch uncouples Akt activation from hepatic lipid accumulation by decreasing mTorc1 stability.
Activation of liver X receptors reduces CFTR-mediated Cl(-) transport in kidney collecting duct cells.
Nuclear transport modulation reduces hypercholesterolemia, atherosclerosis, and fatty liver.
A novel ChREBP isoform in adipose tissue regulates systemic glucose metabolism.
Regulation of SREBP-1 expression and transcriptional action on HKII and FAS genes during fasting and refeeding in rat tissues.
Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens.
Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins.
Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism.
Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism.
Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins.
Sterol regulatory element-binding protein-1 interacts with the nuclear thyroid hormone receptor to enhance acetyl-CoA carboxylase-alpha transcription in hepatocytes.
Elevated levels of SREBP-2 and cholesterol synthesis in livers of mice homozygous for a targeted disruption of the SREBP-1 gene.
Independent regulation of sterol regulatory element-binding proteins 1 and 2 in hamster liver.
Independent regulation of sterol regulatory element-binding proteins 1 and 2 in hamster liver.
Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory element-binding protein-1 (SREBP-1).
Park YH, An M, Kim JK, Lim YH
Journal of ethnopharmacology 2020 Apr 6;251:112542
Journal of ethnopharmacology 2020 Apr 6;251:112542
Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.
Yang N, Li C, Li H, Liu M, Cai X, Cao F, Feng Y, Li M, Wang X
Frontiers in pharmacology 2019;10:709
Frontiers in pharmacology 2019;10:709
The MYC Oncogene Cooperates with Sterol-Regulated Element-Binding Protein to Regulate Lipogenesis Essential for Neoplastic Growth.
Gouw AM, Margulis K, Liu NS, Raman SJ, Mancuso A, Toal GG, Tong L, Mosley A, Hsieh AL, Sullivan DK, Stine ZE, Altman BJ, Schulze A, Dang CV, Zare RN, Felsher DW
Cell metabolism 2019 Sep 3;30(3):556-572.e5
Cell metabolism 2019 Sep 3;30(3):556-572.e5
Combined Exposure to Fructose and Bisphenol A Exacerbates Abnormal Lipid Metabolism in Liver of Developmental Male Rats.
Lin R, Jia Y, Wu F, Meng Y, Sun Q, Jia L
International journal of environmental research and public health 2019 Oct 28;16(21)
International journal of environmental research and public health 2019 Oct 28;16(21)
Benzyl Isothiocyanate and Phenethyl Isothiocyanate Inhibit Adipogenesis and Hepatosteatosis in Mice with Obesity Induced by a High-Fat Diet.
Chuang WT, Liu YT, Huang CS, Lo CW, Yao HT, Chen HW, Lii CK
Journal of agricultural and food chemistry 2019 Jun 26;67(25):7136-7146
Journal of agricultural and food chemistry 2019 Jun 26;67(25):7136-7146
Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells.
Pham DD, Bruelle C, Thi Do H, Pajanoja C, Jin C, Srinivasan V, Olkkonen VM, Eriksson O, Jauhiainen M, Lalowski M, Lindholm D
Cell death & disease 2019 Jul 11;10(7):537
Cell death & disease 2019 Jul 11;10(7):537
Hepatic Forkhead Box Protein A3 Regulates ApoA-I (Apolipoprotein A-I) Expression, Cholesterol Efflux, and Atherogenesis.
Li Y, Xu Y, Jadhav K, Zhu Y, Yin L, Zhang Y
Arteriosclerosis, thrombosis, and vascular biology 2019 Aug;39(8):1574-1587
Arteriosclerosis, thrombosis, and vascular biology 2019 Aug;39(8):1574-1587
Inhibition of Hepatotoxicity by a LXR Inverse Agonist in a Model of Alcoholic Liver Disease.
Sengupta M, Griffett K, Flaveny CA, Burris TP
ACS pharmacology & translational science 2018 Sep 14;1(1):50-60
ACS pharmacology & translational science 2018 Sep 14;1(1):50-60
14-Deoxy-11,12-didehydroandrographolide suppresses adipogenesis of 3 T3-L1 preadipocytes by inhibiting CCAAT/enhancer-binding protein β activation and AMPK-mediated mitotic clonal expansion.
Li CC, Yen CC, Fan CT, Chuang WT, Huang CS, Chen HW, Lii CK
Toxicology and applied pharmacology 2018 Nov 15;359:82-90
Toxicology and applied pharmacology 2018 Nov 15;359:82-90
Phytoceuticals in Fenugreek Ameliorate VLDL Overproduction and Insulin Resistance via the Insig Signaling Pathway.
Khound R, Shen J, Song Y, Santra D, Su Q
Molecular nutrition & food research 2018 Mar;62(5)
Molecular nutrition & food research 2018 Mar;62(5)
Establishment and analysis of a novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation.
Ikenoue T, Terakado Y, Zhu C, Liu X, Ohsugi T, Matsubara D, Fujii T, Kakuta S, Kubo S, Shibata T, Yamaguchi K, Iwakura Y, Furukawa Y
Scientific reports 2018 Jan 31;8(1):2021
Scientific reports 2018 Jan 31;8(1):2021
Angiotensin II induces cholesterol accumulation and injury in podocytes.
Yang Y, Yang Q, Yang J, Ma Y, Ding G
Scientific reports 2017 Sep 6;7(1):10672
Scientific reports 2017 Sep 6;7(1):10672
An In Vivo Magnetic Resonance Spectroscopy Study of the Effects of Caloric and Non-Caloric Sweeteners on Liver Lipid Metabolism in Rats.
Janssens S, Ciapaite J, Wolters JC, van Riel NA, Nicolay K, Prompers JJ
Nutrients 2017 May 10;9(5)
Nutrients 2017 May 10;9(5)
Telomerase reverse transcriptase (TERT) - enhancer of zeste homolog 2 (EZH2) network regulates lipid metabolism and DNA damage responses in glioblastoma.
Ahmad F, Patrick S, Sheikh T, Sharma V, Pathak P, Malgulwar PB, Kumar A, Joshi SD, Sarkar C, Sen E
Journal of neurochemistry 2017 Dec;143(6):671-683
Journal of neurochemistry 2017 Dec;143(6):671-683
Lack of mitochondria-generated acetyl-CoA by pyruvate dehydrogenase complex downregulates gene expression in the hepatic de novo lipogenic pathway.
Mahmood S, Birkaya B, Rideout TC, Patel MS
American journal of physiology. Endocrinology and metabolism 2016 Jul 1;311(1):E117-27
American journal of physiology. Endocrinology and metabolism 2016 Jul 1;311(1):E117-27
Hyperactive mTORC1 signaling is unaffected by metformin treatment in aged skeletal muscle.
Dungan CM, Li Z, Wright DC, Williamson DL
Muscle & nerve 2016 Jan;53(1):107-17
Muscle & nerve 2016 Jan;53(1):107-17
Caloric Restriction Normalizes Obesity-Induced Alterations on Regulators of Skeletal Muscle Growth Signaling.
Dungan CM, Li J, Williamson DL
Lipids 2016 Aug;51(8):905-12
Lipids 2016 Aug;51(8):905-12
MicroRNA-148a regulates LDL receptor and ABCA1 expression to control circulating lipoprotein levels.
Goedeke L, Rotllan N, Canfrán-Duque A, Aranda JF, Ramírez CM, Araldi E, Lin CS, Anderson NN, Wagschal A, de Cabo R, Horton JD, Lasunción MA, Näär AM, Suárez Y, Fernández-Hernando C
Nature medicine 2015 Nov;21(11):1280-9
Nature medicine 2015 Nov;21(11):1280-9
Omega-3 Fatty Acids Augment the Actions of Nuclear Receptor Agonists in a Mouse Model of Alzheimer's Disease.
Casali BT, Corona AW, Mariani MM, Karlo JC, Ghosal K, Landreth GE
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jun 17;35(24):9173-81
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jun 17;35(24):9173-81
Phytosterols protect against diet-induced hypertriglyceridemia in Syrian golden hamsters.
Rideout TC, Ramprasath V, Griffin JD, Browne RW, Harding SV, Jones PJ
Lipids in health and disease 2014 Jan 6;13:5
Lipids in health and disease 2014 Jan 6;13:5
Alpha-lipoic acid supplementation reduces mTORC1 signaling in skeletal muscle from high fat fed, obese Zucker rats.
Li Z, Dungan CM, Carrier B, Rideout TC, Williamson DL
Lipids 2014 Dec;49(12):1193-201
Lipids 2014 Dec;49(12):1193-201
Treadmill Exercise Training Modulates Hepatic Cholesterol Metabolism and Circulating PCSK9 Concentration in High-Fat-Fed Mice.
Wen S, Jadhav KS, Williamson DL, Rideout TC
Journal of lipids 2013;2013:908048
Journal of lipids 2013;2013:908048
Inhibition of Notch uncouples Akt activation from hepatic lipid accumulation by decreasing mTorc1 stability.
Pajvani UB, Qiang L, Kangsamaksin T, Kitajewski J, Ginsberg HN, Accili D
Nature medicine 2013 Aug;19(8):1054-60
Nature medicine 2013 Aug;19(8):1054-60
Activation of liver X receptors reduces CFTR-mediated Cl(-) transport in kidney collecting duct cells.
Raksaseri P, Chatsudthipong V, Muanprasat C, Soodvilai S
American journal of physiology. Renal physiology 2013 Aug 15;305(4):F583-91
American journal of physiology. Renal physiology 2013 Aug 15;305(4):F583-91
Nuclear transport modulation reduces hypercholesterolemia, atherosclerosis, and fatty liver.
Liu Y, Major AS, Zienkiewicz J, Gabriel CL, Veach RA, Moore DJ, Collins RD, Hawiger J
Journal of the American Heart Association 2013 Apr 5;2(2):e000093
Journal of the American Heart Association 2013 Apr 5;2(2):e000093
A novel ChREBP isoform in adipose tissue regulates systemic glucose metabolism.
Herman MA, Peroni OD, Villoria J, Schön MR, Abumrad NA, Blüher M, Klein S, Kahn BB
Nature 2012 Apr 19;484(7394):333-8
Nature 2012 Apr 19;484(7394):333-8
Regulation of SREBP-1 expression and transcriptional action on HKII and FAS genes during fasting and refeeding in rat tissues.
Gosmain Y, Dif N, Berbe V, Loizon E, Rieusset J, Vidal H, Lefai E
Journal of lipid research 2005 Apr;46(4):697-705
Journal of lipid research 2005 Apr;46(4):697-705
Starvation and feeding a high-carbohydrate, low-fat diet regulate the expression sterol regulatory element-binding protein-1 in chickens.
Zhang Y, Hillgartner FB
The Journal of nutrition 2004 Sep;134(9):2205-10
The Journal of nutrition 2004 Sep;134(9):2205-10
Platelet-derived growth factor stimulates membrane lipid synthesis through activation of phosphatidylinositol 3-kinase and sterol regulatory element-binding proteins.
Demoulin JB, Ericsson J, Kallin A, Rorsman C, Rönnstrand L, Heldin CH
The Journal of biological chemistry 2004 Aug 20;279(34):35392-402
The Journal of biological chemistry 2004 Aug 20;279(34):35392-402
Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism.
Kim HJ, Miyazaki M, Ntambi JM
Journal of lipid research 2002 Oct;43(10):1750-7
Journal of lipid research 2002 Oct;43(10):1750-7
Dietary cholesterol opposes PUFA-mediated repression of the stearoyl-CoA desaturase-1 gene by SREBP-1 independent mechanism.
Kim HJ, Miyazaki M, Ntambi JM
Journal of lipid research 2002 Oct;43(10):1750-7
Journal of lipid research 2002 Oct;43(10):1750-7
Insig-2, a second endoplasmic reticulum protein that binds SCAP and blocks export of sterol regulatory element-binding proteins.
Yabe D, Brown MS, Goldstein JL
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12753-8
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 1;99(20):12753-8
Sterol regulatory element-binding protein-1 interacts with the nuclear thyroid hormone receptor to enhance acetyl-CoA carboxylase-alpha transcription in hepatocytes.
Yin L, Zhang Y, Hillgartner FB
The Journal of biological chemistry 2002 May 31;277(22):19554-65
The Journal of biological chemistry 2002 May 31;277(22):19554-65
Elevated levels of SREBP-2 and cholesterol synthesis in livers of mice homozygous for a targeted disruption of the SREBP-1 gene.
Shimano H, Shimomura I, Hammer RE, Herz J, Goldstein JL, Brown MS, Horton JD
The Journal of clinical investigation 1997 Oct 15;100(8):2115-24
The Journal of clinical investigation 1997 Oct 15;100(8):2115-24
Independent regulation of sterol regulatory element-binding proteins 1 and 2 in hamster liver.
Sheng Z, Otani H, Brown MS, Goldstein JL
Proceedings of the National Academy of Sciences of the United States of America 1995 Feb 14;92(4):935-8
Proceedings of the National Academy of Sciences of the United States of America 1995 Feb 14;92(4):935-8
Independent regulation of sterol regulatory element-binding proteins 1 and 2 in hamster liver.
Sheng Z, Otani H, Brown MS, Goldstein JL
Proceedings of the National Academy of Sciences of the United States of America 1995 Feb 14;92(4):935-8
Proceedings of the National Academy of Sciences of the United States of America 1995 Feb 14;92(4):935-8
Assignment of the membrane attachment, DNA binding, and transcriptional activation domains of sterol regulatory element-binding protein-1 (SREBP-1).
Sato R, Yang J, Wang X, Evans MJ, Ho YK, Goldstein JL, Brown MS
The Journal of biological chemistry 1994 Jun 24;269(25):17267-73
The Journal of biological chemistry 1994 Jun 24;269(25):17267-73
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Analysis of whole cell lysate from HeLa cells showing a single specific band for the expression of SREBP1 precursor protein (~120 kDa).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY licence.
