AP1228a

antibody from Abcepta

Targeting: UBC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP1228a

Provider

Abcepta

Proper citation

Abgent Cat#AP1228a, RRID:AB_352629

Product name

Ubiquitin Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of anti-Ubiquitin Pab (Cat. #AP1228a) in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Ubiquitin antibody (N-term) (Cat.#AP1228a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human Kidney tissue reacted with Ubiquitin antibody (N-term) (Cat.#AP1228a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100