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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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- Product number
- F42055 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Ubiquitin Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1-32 from the human protein was used as the immunogen for this anti-Ubiquitin antibody.
- Description
- Purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Can be stored at 4°C for one month. For long-term, aliquot the anti-Ubiquitin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of anti-Ubiquitin antibody and HeLa cell lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human 293, 2) human HeLa, 3) human HepG2, 4) human A549, 5) mouse NIH 3T3, 6) rat C2C12 and 7) rat C6 cell lysate with anti-Ubiquitin antibody. Predicted molecular weight ~8 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with anti-Ubiquitin antibody
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human kidney tissue stained with anti-Ubiquitin antibody
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human breast carcinoma with anti-Ubiquitin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human kidney tissue with anti-Ubiquitin antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
