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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA3-16717 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Ubiquitin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inhibition of Ubiquitin-Specific Protease-13 Improves Behavioral Performance in Alpha-Synuclein Expressing Mice.
Genetic analysis reveals functions of atypical polyubiquitin chains.
Liu X, Balaraman K, Lynch CC, Hebron M, Shah PK, Hu S, Stevenson M, Wolf C, Moussa C
International journal of molecular sciences 2022 Jul 23;23(15)
International journal of molecular sciences 2022 Jul 23;23(15)
Genetic analysis reveals functions of atypical polyubiquitin chains.
Meza Gutierrez F, Simsek D, Mizrak A, Deutschbauer A, Braberg H, Johnson J, Xu J, Shales M, Nguyen M, Tamse-Kuehn R, Palm C, Steinmetz LM, Krogan NJ, Toczyski DP
eLife 2018 Dec 14;7
eLife 2018 Dec 14;7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Ubiquitin in HEK293 cell lysates. Samples were incubated in Ubiquitin polyclonal antibody (Product # PA3-16717 using a dilution of 1:5000. Ubiquitin antibody and mouse beta-Tubulin antibody, dilution 1:10000 (Red) used as a loading control. [1] protein standard (Red), [2] cells maintained in normal medium, [3] cells treated with proteasome inhibitor lactacystin (Lc) at 10 µM for 16 hours. Lysed cells were lysed and the lysate subjected to electrophoresis on a 4-20% SDS-PAGE gel, then electrophoretically transferred to PVDF membranes. The smear detected above the 200 kDa standard represents accumulations of ubiquitinated proteins in the Lc treated cells. The prominent band at ~8 kDa corresponds to monoubiquitin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Ubiquitin in HeLa cells. Samples were incubated in Ubiquitin polyclonal antibody (Product # PA3-16717) using a dilution of 1:1000. Ubiquitin (red) costained with chicken pAb to vimentin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. [A] Control HeLa cells maintained in normal medium, [B] HeLa cells treated with 10 µM of the proteasome inhibitor lactacystin (Lc) for 24 hours. Proteasomal inhibition leads to formation of strongly ubiquitin positive cytoplasmic inclusions. Note the diffuse cytoplasmic ubiquitin staining in control cells and well defined ubiquitin positive inclusions in the Lc treated cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Ubiquitin in aggresomes of mouse L-cells treated with lactacysteine. Samples were incubated in Ubiquitin polyclonal antibody (Product # PA3-16717).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Ubiquitin in human rectal cancer. Samples were incubated in Ubiquitin polyclonal antibody (Product # PA3-16717) followed by DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1--figure supplement 2. Analysis of ubiquitin levels in K-to-R ubiquitin mutants. The levels of ubiquitin were analyzed by western blotting. A wild-type SK1 strain with endogenous UBI1-4 was compared to the strains used in this study that express K-to-R mutant ubiquitin alleles, as well as wild-type ubiquitin, from engineered ubiquitin loci. PSTAIR was used as loading control.
