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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BML-PW1225-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-PW1225-0025, RRID:AB_11179654
- Product name
- Ubiquitin monoclonal antibody (EX-9) (fluorescein labeled)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Ion exchange purified.
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- EX-9
- Vial size
- 25 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Detection of ubiquitinylated protein conjugates in HeLa cells by fluorescence microscopy using fluorescein labeled EX-9 mAb (BML-PW1225), at 1:250 dilution. HeLa cells were treated with proteasome inhibitor MG-132 (5µM, BML-PI102) for 12 hours prior to analysis.
