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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-PW0150-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) (HRP conjugate)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Host
- Mouse
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Antibody clone number
- FK2
- Vial size
- 100 μg
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references PI3K/AKT activation induces PTEN ubiquitination and destabilization accelerating tumourigenesis.
Efficient APC/C substrate degradation in cells undergoing mitotic exit depends on K11 ubiquitin linkages.
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.
Selective degradation of aggregate-prone CryAB mutants by HSPB1 is mediated by ubiquitin-proteasome pathways.
Lee MS, Jeong MH, Lee HW, Han HJ, Ko A, Hewitt SM, Kim JH, Chun KH, Chung JY, Lee C, Cho H, Song J
Nature communications 2015 Jul 17;6:7769
Nature communications 2015 Jul 17;6:7769
Efficient APC/C substrate degradation in cells undergoing mitotic exit depends on K11 ubiquitin linkages.
Min M, Mevissen TE, De Luca M, Komander D, Lindon C
Molecular biology of the cell 2015 Dec 1;26(24):4325-32
Molecular biology of the cell 2015 Dec 1;26(24):4325-32
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.
Sarkar AA, Zohn IE
The Journal of cell biology 2012 Mar 19;196(6):789-800
The Journal of cell biology 2012 Mar 19;196(6):789-800
Selective degradation of aggregate-prone CryAB mutants by HSPB1 is mediated by ubiquitin-proteasome pathways.
Zhang H, Rajasekaran NS, Orosz A, Xiao X, Rechsteiner M, Benjamin IJ
Journal of molecular and cellular cardiology 2010 Dec;49(6):918-30
Journal of molecular and cellular cardiology 2010 Dec;49(6):918-30
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Lanes a-d, HeLa S3 S100 cytosol preparation (SW8750; 10µg per lane); lanes e-h, K48-linked multiubiquitin chains (Prod. No: BML-PW8860; 0.5µg per lane). Lanes a & e probed with Prod. No BML-PW8805 (clone FK1; 1µg/mL); lanes b & f probed with Prod. No. BML-PW0145 (FK1Hª; 1µg/mL); lanes c & g probed withÊProd. No. BML-PW8810 (clone FK2; 1µg/mL); lanes d & h probed with Prod. No. BML-PW0150 (FK2Hª; 1µg/mL).
