ADI-SPA-203-D

antibody from Enzo Life Sciences

Targeting: UBC

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

ADI-SPA-203-D

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-SPA-203-D, RRID:AB_2039665

Product name

Ubiquitin, mAb (P4G7-H11)

Antibody type

Monoclonal

Antigen

Other

Description

Protein G affinity purified. Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.

Isotype

IgG

Antibody clone number

P4G7-H11

References

Submitted references

ERK-dependent threonine phosphorylation of EGF receptor modulates receptor downregulation and signaling.

Li X, Huang Y, Jiang J, Frank SJ
Cellular signalling 2008 Nov;20(11):2145-55

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of Ubiquitin: Lane 1: MW marker, Lane 2: Ubiquitin, Lane 3: HeLa, Lane 4: RK-13, Lane 5: 3T3, Lane 6: PC-12, Lane 7: Mouse Brain, Lane 8: Rat Brain, Lane 9: MDBK

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis of human brain, cortex tissue stained with Ubiquitin, mAb (P4G7-H11) at 5-10µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Flow cytometry analysis of human hepatoma QGY cells analyzed by flow cytometry using isotype control antibody (left) or Ubiquitin Monoclonal Antibody (P4G7-H11) (right) at 10µg/mL