BML-PW0605-0025

antibody from Enzo Life Sciences

Targeting: UBC

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

BML-PW0605-0025

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#BML-PW0605-0025, RRID:AB_2052279

Product name

Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4) (HRP conjugate)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Protein G-affinity purified.

Host

Mouse

Conjugate

Horseradish Peroxidase

Isotype

IgG

Antibody clone number

HWA4C4

Vial size

25 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength.

Zhang P, Fu WY, Fu AK, Ip NY
Nature communications 2015 Oct 27;6:8665

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot of single isopeptide-linkage polyubiquitinylated conjugates (K6 - Prod. No. BML-UW0615, K11 - Prod. No. BML-UW0620, K27 - Prod. No. BML-UW0625, K29 - Prod. No. BML-UW0630, K33 - Prod. No. BML-UW0635, K48 - Prod. No. BML-UW0640, K63 - Prod. No. BML-UW0645, 1µg)Êusing MAb to Polyubiquitinylated Conjugates (FK1)Ê(Prod.ÊNo. BML-PW8805)Êand MAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) (HRP) (Prod. No. BML-PW0605) at 1:1'000 dilution.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot of HeLa S100 cytosolic fraction (Prod. No. BML-SW8750, 5µg) probed with K63-linkage specific HRP-conjugateÊand generalÊubiquitin conjugate-reactive monoclonal antibodies at 1:1\\'000 dilution. Lane A. MAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) (HRP) (Prod. No. BML-PW0605). Lane B. MAb to Mono- and Polyubiquitinylated Conjugates (FK2H) (HRP) (Prod. No. BML-PW0150).