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ImmunocytochemistryAntibody data
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- References [1]
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- Validations
- Immunocytochemistry [2]
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- Product number
- BML-PW0605-0100 - Provider product page
- Provider
- Enzo Life Sciences
- Product name
- Polyubiquitin (K63-linkage-specific) monoclonal antibody (HWA4C4) (HRP conjugate)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Protein G-affinity purified.
- Host
- Mouse
- Conjugate
- Horseradish Peroxidase
- Isotype
- IgG
- Antibody clone number
- HWA4C4
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references S-nitrosylation-dependent proteasomal degradation restrains Cdk5 activity to regulate hippocampal synaptic strength.
Zhang P, Fu WY, Fu AK, Ip NY
Nature communications 2015 Oct 27;6:8665
Nature communications 2015 Oct 27;6:8665
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot of single isopeptide-linkage polyubiquitinylated conjugates (K6 - Prod. No. BML-UW0615, K11 - Prod. No. BML-UW0620, K27 - Prod. No. BML-UW0625, K29 - Prod. No. BML-UW0630, K33 - Prod. No. BML-UW0635, K48 - Prod. No. BML-UW0640, K63 - Prod. No. BML-UW0645, 1µg)Êusing MAb to Polyubiquitinylated Conjugates (FK1)Ê(Prod.ÊNo. BML-PW8805)Êand MAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) (HRP) (Prod. No. BML-PW0605) at 1:1'000 dilution.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot of HeLa S100 cytosolic fraction (Prod. No. BML-SW8750, 5µg) probed with K63-linkage specific HRP-conjugateÊand generalÊubiquitin conjugate-reactive monoclonal antibodies at 1:1\\'000 dilution. Lane A. MAb to Polyubiquitin (K63-linkage-specific) (HWA4C4) (HRP) (Prod. No. BML-PW0605). Lane B. MAb to Mono- and Polyubiquitinylated Conjugates (FK2H) (HRP) (Prod. No. BML-PW0150).
