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- Antibody Data
- Antigen structure
- References [24]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-PW8805-0500 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-PW8805-0500, RRID:AB_2052280
- Product name
- Polyubiquitinylated conjugates monoclonal antibody (FK1)
- Antibody type
- Monoclonal
- Antigen
- Purified from natural sources
- Description
- Purified.
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- FK1
- Vial size
- 500 μg
- Storage
- +4°C
- Handling
- Store at 4¡C when not in use. Use diluted antibody within 1 month. Do not freeze.
Submitted references Effectiveness of daily eccentric contractions induced via kilohertz frequency transcutaneous electrical stimulation on muscle atrophy.
A voltage-gated calcium channel regulates lysosomal fusion with endosomes and autophagosomes and is required for neuronal homeostasis.
Structure of the Legionella Virulence Factor, SidC Reveals a Unique PI(4)P-Specific Binding Domain Essential for Its Targeting to the Bacterial Phagosome.
Particulate cytoplasmic structures with high concentration of ubiquitin-proteasome accumulate in myeloid neoplasms.
Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of β2 adrenergic receptors to autophagosomes during physiological stress.
Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions.
Ubiquitin-dependent recruitment of the Bloom syndrome helicase upon replication stress is required to suppress homologous recombination.
Mitochondrial quality, dynamics and functional capacity in Parkinson's disease cybrid cell lines selected for Lewy body expression.
Activation of the cAMP/PKA pathway induces UT-A1 urea transporter monoubiquitination and targets it for lysosomal degradation.
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.
NF-κB activation and polyubiquitin conjugation are required for pulmonary inflammation-induced diaphragm atrophy.
53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress.
Fibroblast growth factor receptor 3 (FGFR3) is a strong heat shock protein 90 (Hsp90) client: implications for therapeutic manipulation.
Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity.
p62 and NDP52 proteins target intracytosolic Shigella and Listeria to different autophagy pathways.
p23H implicated as cis/trans regulator of AlaXp-directed editing for mammalian cell homeostasis.
PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.
Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation.
The FMR1 CGG repeat mouse displays ubiquitin-positive intranuclear neuronal inclusions; implications for the cerebellar tremor/ataxia syndrome.
PI31 is a modulator of proteasome formation and antigen processing.
Transient aggregation of ubiquitinated proteins during dendritic cell maturation.
Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains.
Immunoassay for the quantification of intracellular multi-ubiquitin chains.
Tanaka M, Nakanishi R, Murakami S, Fujita N, Kondo H, Ishihara A, Roy RR, Fujino H
Acta histochemica 2016 Jan;118(1):56-62
Acta histochemica 2016 Jan;118(1):56-62
A voltage-gated calcium channel regulates lysosomal fusion with endosomes and autophagosomes and is required for neuronal homeostasis.
Tian X, Gala U, Zhang Y, Shang W, Nagarkar Jaiswal S, di Ronza A, Jaiswal M, Yamamoto S, Sandoval H, Duraine L, Sardiello M, Sillitoe RV, Venkatachalam K, Fan H, Bellen HJ, Tong C
PLoS biology 2015 Mar;13(3):e1002103
PLoS biology 2015 Mar;13(3):e1002103
Structure of the Legionella Virulence Factor, SidC Reveals a Unique PI(4)P-Specific Binding Domain Essential for Its Targeting to the Bacterial Phagosome.
Luo X, Wasilko DJ, Liu Y, Sun J, Wu X, Luo ZQ, Mao Y
PLoS pathogens 2015 Jun;11(6):e1004965
PLoS pathogens 2015 Jun;11(6):e1004965
Particulate cytoplasmic structures with high concentration of ubiquitin-proteasome accumulate in myeloid neoplasms.
Pecci A, Necchi V, Barozzi S, Vitali A, Boveri E, Elena C, Bernasconi P, Noris P, Solcia E
Journal of hematology & oncology 2015 Jun 18;8:71
Journal of hematology & oncology 2015 Jun 18;8:71
Phosphorylation of the deubiquitinase USP20 by protein kinase A regulates post-endocytic trafficking of β2 adrenergic receptors to autophagosomes during physiological stress.
Kommaddi RP, Jean-Charles PY, Shenoy SK
The Journal of biological chemistry 2015 Apr 3;290(14):8888-903
The Journal of biological chemistry 2015 Apr 3;290(14):8888-903
Sestrin 2 protein regulates platelet-derived growth factor receptor β (Pdgfrβ) expression by modulating proteasomal and Nrf2 transcription factor functions.
Tomasovic A, Kurrle N, Sürün D, Heidler J, Husnjak K, Poser I, Schnütgen F, Scheibe S, Seimetz M, Jaksch P, Hyman A, Weissmann N, von Melchner H
The Journal of biological chemistry 2015 Apr 10;290(15):9738-52
The Journal of biological chemistry 2015 Apr 10;290(15):9738-52
Ubiquitin-dependent recruitment of the Bloom syndrome helicase upon replication stress is required to suppress homologous recombination.
Tikoo S, Madhavan V, Hussain M, Miller ES, Arora P, Zlatanou A, Modi P, Townsend K, Stewart GS, Sengupta S
The EMBO journal 2013 Jun 12;32(12):1778-92
The EMBO journal 2013 Jun 12;32(12):1778-92
Mitochondrial quality, dynamics and functional capacity in Parkinson's disease cybrid cell lines selected for Lewy body expression.
Cronin-Furman EN, Borland MK, Bergquist KE, Bennett JP Jr, Trimmer PA
Molecular neurodegeneration 2013 Jan 26;8:6
Molecular neurodegeneration 2013 Jan 26;8:6
Activation of the cAMP/PKA pathway induces UT-A1 urea transporter monoubiquitination and targets it for lysosomal degradation.
Su H, Chen M, Sands JM, Chen G
American journal of physiology. Renal physiology 2013 Dec 15;305(12):F1775-82
American journal of physiology. Renal physiology 2013 Dec 15;305(12):F1775-82
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme.
Sarkar AA, Zohn IE
The Journal of cell biology 2012 Mar 19;196(6):789-800
The Journal of cell biology 2012 Mar 19;196(6):789-800
NF-κB activation and polyubiquitin conjugation are required for pulmonary inflammation-induced diaphragm atrophy.
Haegens A, Schols AM, Gorissen SH, van Essen AL, Snepvangers F, Gray DA, Shoelson SE, Langen RC
American journal of physiology. Lung cellular and molecular physiology 2012 Jan 1;302(1):L103-10
American journal of physiology. Lung cellular and molecular physiology 2012 Jan 1;302(1):L103-10
53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of chromosomes under replication stress.
Lukas C, Savic V, Bekker-Jensen S, Doil C, Neumann B, Pedersen RS, Grøfte M, Chan KL, Hickson ID, Bartek J, Lukas J
Nature cell biology 2011 Mar;13(3):243-53
Nature cell biology 2011 Mar;13(3):243-53
Fibroblast growth factor receptor 3 (FGFR3) is a strong heat shock protein 90 (Hsp90) client: implications for therapeutic manipulation.
Laederich MB, Degnin CR, Lunstrum GP, Holden P, Horton WA
The Journal of biological chemistry 2011 Jun 3;286(22):19597-604
The Journal of biological chemistry 2011 Jun 3;286(22):19597-604
Histone crosstalk directed by H2B ubiquitination is required for chromatin boundary integrity.
Ma MK, Heath C, Hair A, West AG
PLoS genetics 2011 Jul;7(7):e1002175
PLoS genetics 2011 Jul;7(7):e1002175
p62 and NDP52 proteins target intracytosolic Shigella and Listeria to different autophagy pathways.
Mostowy S, Sancho-Shimizu V, Hamon MA, Simeone R, Brosch R, Johansen T, Cossart P
The Journal of biological chemistry 2011 Jul 29;286(30):26987-95
The Journal of biological chemistry 2011 Jul 29;286(30):26987-95
p23H implicated as cis/trans regulator of AlaXp-directed editing for mammalian cell homeostasis.
Nawaz MH, Merriman E, Yang XL, Schimmel P
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 15;108(7):2723-8
Proceedings of the National Academy of Sciences of the United States of America 2011 Feb 15;108(7):2723-8
PINK1/Parkin-mediated mitophagy is dependent on VDAC1 and p62/SQSTM1.
Geisler S, Holmström KM, Skujat D, Fiesel FC, Rothfuss OC, Kahle PJ, Springer W
Nature cell biology 2010 Feb;12(2):119-31
Nature cell biology 2010 Feb;12(2):119-31
Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation.
Haglund K, Sigismund S, Polo S, Szymkiewicz I, Di Fiore PP, Dikic I
Nature cell biology 2003 May;5(5):461-6
Nature cell biology 2003 May;5(5):461-6
The FMR1 CGG repeat mouse displays ubiquitin-positive intranuclear neuronal inclusions; implications for the cerebellar tremor/ataxia syndrome.
Willemsen R, Hoogeveen-Westerveld M, Reis S, Holstege J, Severijnen LA, Nieuwenhuizen IM, Schrier M, van Unen L, Tassone F, Hoogeveen AT, Hagerman PJ, Mientjes EJ, Oostra BA
Human molecular genetics 2003 May 1;12(9):949-59
Human molecular genetics 2003 May 1;12(9):949-59
PI31 is a modulator of proteasome formation and antigen processing.
Zaiss DM, Standera S, Kloetzel PM, Sijts AJ
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 29;99(22):14344-9
Proceedings of the National Academy of Sciences of the United States of America 2002 Oct 29;99(22):14344-9
Transient aggregation of ubiquitinated proteins during dendritic cell maturation.
Lelouard H, Gatti E, Cappello F, Gresser O, Camosseto V, Pierre P
Nature 2002 May 9;417(6885):177-82
Nature 2002 May 9;417(6885):177-82
Mammalian class E vps proteins recognize ubiquitin and act in the removal of endosomal protein-ubiquitin conjugates.
Bishop N, Horman A, Woodman P
The Journal of cell biology 2002 Apr 1;157(1):91-101
The Journal of cell biology 2002 Apr 1;157(1):91-101
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains.
Fujimuro M, Sawada H, Yokosawa H
European journal of biochemistry / FEBS 1997 Oct 15;249(2):427-33
European journal of biochemistry / FEBS 1997 Oct 15;249(2):427-33
Immunoassay for the quantification of intracellular multi-ubiquitin chains.
Takada K, Nasu H, Hibi N, Tsukada Y, Ohkawa K, Fujimuro M, Sawada H, Yokosawa H
European journal of biochemistry / FEBS 1995 Oct 1;233(1):42-7
European journal of biochemistry / FEBS 1995 Oct 1;233(1):42-7
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot of multi-ubiquitin chains using MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) (lanes A-C) and MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8810) (lanes D-F).Lanes A & D: K48-linked chains.Lanes B & E: K29-linked chains. Lanes C & F: K63-linked chains.
