MA5-16429
antibody from Invitrogen Antibodies
Targeting: VCAM1
CD106
Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- MA5-16429 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VCAM-1 Monoclonal Antibody (1.G11B1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Non-reducing conditions are recommended for Western blot applications.
- Reactivity
- Human, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1.G11B1
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The pretubulysin-induced exposure of collagen is caused by endothelial cell retraction that results in an increased adhesion and decreased transmigration of tumor cells.
Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.
Effects of vascular endothelial growth factor on the lymphocyte-endothelium interactions: identification of caveolin-1 and nitric oxide as control points of endothelial cell anergy.
Immunological and ultrastructural characterization of endothelial cell cultures differentiated from human cord blood derived endothelial progenitor cells.
Irradiation of mechanically-injured human arterial endothelial cells leads to increased gene expression and secretion of inflammatory and growth promoting cytokines.
Effect of maternal anti-HPA-1a antibodies and polyclonal IVIG on the activation status of vascular endothelial cells.
Human pulmonary valve endothelial cells express functional adhesion molecules for leukocytes.
Schwenk R, Stehning T, Bischoff I, Ullrich A, Kazmaier U, Fürst R
Oncotarget 2017 Sep 29;8(44):77622-77633
Oncotarget 2017 Sep 29;8(44):77622-77633
Adhesion of human hematopoietic progenitor cells to mesenchymal stromal cells involves CD44.
Wagner W, Wein F, Roderburg C, Saffrich R, Diehlmann A, Eckstein V, Ho AD
Cells, tissues, organs 2008;188(1-2):160-9
Cells, tissues, organs 2008;188(1-2):160-9
Effects of vascular endothelial growth factor on the lymphocyte-endothelium interactions: identification of caveolin-1 and nitric oxide as control points of endothelial cell anergy.
Bouzin C, Brouet A, De Vriese J, Dewever J, Feron O
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1505-11
Journal of immunology (Baltimore, Md. : 1950) 2007 Feb 1;178(3):1505-11
Immunological and ultrastructural characterization of endothelial cell cultures differentiated from human cord blood derived endothelial progenitor cells.
Neumüller J, Neumüller-Guber SE, Lipovac M, Mosgoeller W, Vetterlein M, Pavelka M, Huber J
Histochemistry and cell biology 2006 Dec;126(6):649-64
Histochemistry and cell biology 2006 Dec;126(6):649-64
Irradiation of mechanically-injured human arterial endothelial cells leads to increased gene expression and secretion of inflammatory and growth promoting cytokines.
Wondergem J, Wedekind LE, Bart CI, Chin A, van der Laarse A, Beekhuizen H
Atherosclerosis 2004 Jul;175(1):59-67
Atherosclerosis 2004 Jul;175(1):59-67
Effect of maternal anti-HPA-1a antibodies and polyclonal IVIG on the activation status of vascular endothelial cells.
Radder CM, Beekhuizen H, Kanhai HH, Brand A
Clinical and experimental immunology 2004 Jul;137(1):216-22
Clinical and experimental immunology 2004 Jul;137(1):216-22
Human pulmonary valve endothelial cells express functional adhesion molecules for leukocytes.
Dvorin EL, Jacobson J, Roth SJ, Bischoff J
The Journal of heart valve disease 2003 Sep;12(5):617-24
The Journal of heart valve disease 2003 Sep;12(5):617-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of VCAM-1 was performed using 70% confluent log phase HAEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VCAM-1 Mouse monoclonal Antibody (Product # MA5-16429) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2500 for 1 hour at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of KM-H2 cells stained with Mouse anti Human CD106
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 The enhanced expression of ICAM-1 or VCAM-1 is not responsible for the PT-triggered tumor cell adhesion (A/E) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 12 h. The mRNA expression of ICAM-1 (A) or VCAM-1 (E) was analyzed by qPCR experiments. (B/F) Confluent HUVECs were treated with PT (10, 30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The total protein expression of ICAM-1 (B) or VCAM-1 (F) was determined by western blot analysis. (C/G) Confluent HUVECs were treated with PT (30, 100 nM) or TNFalpha (10 ng/ml) for 24 h. The cell surface expression of ICAM-1 (C) or VCAM-1 (G) was analyzed by flow cytometry. (D/H) Confluent HUVECs were treated with 30 nM PT for 24 h. An ICAM-1 blocking antibody (1, 5, 10 mug/ml) (D) or a VCAM-1 blocking antibody (1, 5, 10 mug/ml) (H) was added for the last 30 min of PT treatment. Fluorescence-labeled MDA cells were added and were allowed to adhere for 10 min. The amount of adherent MDA cells was determined by fluorescence measurements. (A-H) Data are expressed as mean +- SEM. A-C and E-H: n=3, D: n=5. *p 0.05 versus PT alone. TNFalpha was used as positive control and was not included into the statistical analyses.