MA5-11447

antibody from Invitrogen Antibodies

Targeting: VCAM1 CD106

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11447

Provider

Invitrogen Antibodies

Product name

VCAM-1 Monoclonal Antibody (1.4C3)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-11447 targets CD106 in IHC (P, F) and ICC/IF applications and shows reactivity with Human samples. The MA5-11447 immunogen is stimulated human umbilical vein endothelial cells (HUVEC). MA5-11447 has been successfully used in immunohistochemistry analysis of VCAM1 in mouse E16.5 frozen lung sections.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1.4C3

Vial size

500 µL

Concentration

0.2 mg/mL

Storage

4° C

References

Submitted references

Innate Immune Dysfunction in Rosacea Promotes Photosensitivity and Vascular Adhesion Molecule Expression.

Kulkarni NN, Takahashi T, Sanford JA, Tong Y, Gombart AF, Hinds B, Cheng JY, Gallo RL
The Journal of investigative dermatology 2020 Mar;140(3):645-655.e6

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Glucocorticoid regulates mesenchymal cell differentiation required for perinatal lung morphogenesis and function.

Bridges JP, Sudha P, Lipps D, Wagner A, Guo M, Du Y, Brown K, Filuta A, Kitzmiller J, Stockman C, Chen X, Weirauch MT, Jobe AH, Whitsett JA, Xu Y
American journal of physiology. Lung cellular and molecular physiology 2020 Aug 1;319(2):L239-L255

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Acute Graft-vs.-Host Disease-Associated Endothelial Activation in vitro Is Prevented by Defibrotide.

Martinez-Sanchez J, Hamelmann H, Palomo M, Mir E, Moreno-Castaño AB, Torramade S, Rovira M, Escolar G, Cordes S, Kalupa M, Mertlitz S, Riesner K, Carreras E, Penack O, Diaz-Ricart M
Frontiers in immunology 2019;10:2339

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Follicular Stimulating Hormone Accelerates Atherogenesis by Increasing Endothelial VCAM-1 Expression.

Li X, Chen W, Li P, Wei J, Cheng Y, Liu P, Yan Q, Xu X, Cui Y, Gu Z, Simoncini T, Fu X
Theranostics 2017;7(19):4671-4688

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Inhibition of cell surface expression of endothelial adhesion molecules by ursolic acid prevents intimal hyperplasia of venous bypass grafts in rats.

Zeller I, Wiedemann D, Schwaiger S, Stelzmüller M, Kreutmayer S, Leberfing O, Stuppner H, Bernhard D
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery 2012 Nov;42(5):878-84

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Identification and pharmacological characterization of the anti-inflammatory principal of the leaves of dwarf elder (Sambucus ebulus L.).

Schwaiger S, Zeller I, Pölzelbauer P, Frotschnig S, Laufer G, Messner B, Pieri V, Stuppner H, Bernhard D
Journal of ethnopharmacology 2011 Jan 27;133(2):704-9

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Leoligin, the major lignan from Edelweiss, inhibits intimal hyperplasia of venous bypass grafts.

Reisinger U, Schwaiger S, Zeller I, Messner B, Stigler R, Wiedemann D, Mayr T, Seger C, Schachner T, Dirsch VM, Vollmar AM, Bonatti JO, Stuppner H, Laufer G, Bernhard D
Cardiovascular research 2009 Jun 1;82(3):542-9

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Immunophenotypic profile and role of adhesion molecules in splenic marginal zone lymphoma with bone marrow involvement.

Florena AM, Tripodo C, Porcasi R, Ingrao S, Fadda MR, De Cantis S, Iannitto E, Franco V
Leukemia & lymphoma 2006 Jan;47(1):49-57

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm of HUVEC cells treated with human TNF-alpha (right) compared to untreated HUVEC cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 monoclonal antibody (Product # MA5-11447) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with CD106 antibody using peroxidase-conjugate and AEC chromogen. Note membrane staining of hematopoietic cells in germinal center.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of VCAM1 in 4% PFA, OCT embedded frozen mouse E16.5 lung sections. Sections were blocked and probed with a VCAM1 mouse monoclonal antibody (Product # MA5-11447) at a dilution of 1:1000. Detection was performed using an Alexa Fluor-568-conjugated anti-mouse secondary antibody (Product # A-11004).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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