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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- Ab133968 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- VCAM1 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit anti Human VCAM1 Antibody, Polyclonal, could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100ICC/IF: 1/50-1/200Protein Function:Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 726-739- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- VCAM1 Antibody (Ab133968) - Western Blot All lanes: VCAM1 Antibody (Ab133968) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 81 kDa Observed band size: 103 kDa Exposure time: 52.9 s
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- VCAM1 Antibody (Ab133968) - Western Blot All lanes: VCAM1 Antibody (Ab133968) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 81 kDa Observed band size: 90 kDa Exposure time: 15.9 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- VCAM1 Antibody (Ab133968) - IF/ICC Immunofluorescence analysis of VCAM1 in K562 using VCAM1 Antibody (Ab133968), and cells were counterstained with DAPI (blue).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- VCAM1 Antibody (Ab133968) - IHC Immunohistochemistry analysis of paraffin-embedded Human tonsil using VCAM1 Antibody (Ab133968). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
