Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AF809 - Provider product page
- Provider
- R&D Systems
- Product name
- Human VCAM-1/CD106 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects VCAM-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse VCAM-1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references N-octanoyl-dopamine is an agonist at the capsaicin receptor TRPV1 and mitigates ischemia-induced [corrected] acute kidney injury in rat.
Mesenchymal stem cells transmigrate between and directly through tumor necrosis factor-α-activated endothelial cells via both leukocyte-like and novel mechanisms.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
Tsagogiorgas C, Wedel J, Hottenrott M, Schneider MO, Binzen U, Greffrath W, Treede RD, Theisinger B, Theisinger S, Waldherr R, Krämer BK, Thiel M, Schnuelle P, Yard BA, Hoeger S
PloS one 2012;7(8):e43525
PloS one 2012;7(8):e43525
Mesenchymal stem cells transmigrate between and directly through tumor necrosis factor-α-activated endothelial cells via both leukocyte-like and novel mechanisms.
Teo GS, Ankrum JA, Martinelli R, Boetto SE, Simms K, Sciuto TE, Dvorak AM, Karp JM, Carman CV
Stem cells (Dayton, Ohio) 2012 Nov;30(11):2472-86
Stem cells (Dayton, Ohio) 2012 Nov;30(11):2472-86
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis and rheumatism 2007 Jun;56(6):1817-26
Arthritis and rheumatism 2007 Jun;56(6):1817-26
Migration inhibitory factor up-regulates vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 via Src, PI3 kinase, and NFkappaB.
Amin MA, Haas CS, Zhu K, Mansfield PJ, Kim MJ, Lackowski NP, Koch AE
Blood 2006 Mar 15;107(6):2252-61
Blood 2006 Mar 15;107(6):2252-61
A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
Carman CV, Springer TA
The Journal of cell biology 2004 Oct 25;167(2):377-88
The Journal of cell biology 2004 Oct 25;167(2):377-88
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human VCAM-1/CD106 by Western Blot. Western blot shows lysates of 786-O human renal cell adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human VCAM-1/ CD106 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF809) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VCAM-1/CD106 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.