Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [32]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BBA5 - Provider product page
- Provider
- R&D Systems
- Product name
- Human VCAM-1/CD106 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. The antibody was screened using COS cells transfected with cDNAs for E-Selectin, VCAM-1, ICAM-1 and PECAM-1 and was shown to be specific for human VCAM-1/CD106.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- BBIG-V1
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Fluoxetine induces direct inhibitory effects on mesenchymal stem cell‑derived osteoprogenitor cells independent of serotonin concentration.
Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression.
Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation.
Lineage dynamics of murine pancreatic development at single-cell resolution.
Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells.
Engineering a multicellular vascular niche to model hematopoietic cell trafficking.
Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium.
Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks.
Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate.
Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii.
Tandem CAR T cells targeting HER2 and IL13Rα2 mitigate tumor antigen escape.
p38 MAP kinase inhibitor suppresses transforming growth factor-β2-induced type 1 collagen production in trabecular meshwork cells.
West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
Uveal melanoma cells utilize a novel route for transendothelial migration.
A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.
IL-15 expression on RA synovial fibroblasts promotes B cell survival.
Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies.
Involvement of native TRPC3 proteins in ATP-dependent expression of VCAM-1 and monocyte adherence in coronary artery endothelial cells.
SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes.
Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated.
Koura SM, Salama M, El-Hussiny M, Khalil MEA, Lotfy A, Hassan SA, Gad Elhak SA, Sobh MA
Molecular medicine reports 2019 Apr;19(4):2611-2619
Molecular medicine reports 2019 Apr;19(4):2611-2619
Leishmania braziliensis Subverts Necroptosis by Modulating RIPK3 Expression.
Luz NF, Khouri R, Van Weyenbergh J, Zanette DL, Fiuza PP, Noronha A, Barral A, Boaventura VS, Prates DB, Chan FK, Andrade BB, Borges VM
Frontiers in microbiology 2018;9:2283
Frontiers in microbiology 2018;9:2283
Stevioside Prevents Wear Particle-Induced Osteolysis by Inhibiting Osteoclastogenesis and Inflammatory Response via the Suppression of TAK1 Activation.
Meng J, Zhou C, Hu B, Luo M, Yang Y, Wang Y, Wang W, Jiang G, Hong J, Li S, Wu H, Yan S, Yan W
Frontiers in pharmacology 2018;9:1053
Frontiers in pharmacology 2018;9:1053
Lineage dynamics of murine pancreatic development at single-cell resolution.
Byrnes LE, Wong DM, Subramaniam M, Meyer NP, Gilchrist CL, Knox SM, Tward AD, Ye CJ, Sneddon JB
Nature communications 2018 Sep 25;9(1):3922
Nature communications 2018 Sep 25;9(1):3922
Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells.
Lager AM, Corradin OG, Cregg JM, Elitt MS, Shick HE, Clayton BLL, Allan KC, Olsen HE, Madhavan M, Tesar PJ
Nature communications 2018 Sep 13;9(1):3708
Nature communications 2018 Sep 13;9(1):3708
Engineering a multicellular vascular niche to model hematopoietic cell trafficking.
Kotha SS, Hayes BJ, Phong KT, Redd MA, Bomsztyk K, Ramakrishnan A, Torok-Storb B, Zheng Y
Stem cell research & therapy 2018 Mar 23;9(1):77
Stem cell research & therapy 2018 Mar 23;9(1):77
Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium.
Lam FW, Da Q, Guillory B, Cruz MA
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1718-1726
Journal of immunology (Baltimore, Md. : 1950) 2018 Mar 1;200(5):1718-1726
Calcium oscillations coordinate feather mesenchymal cell movement by SHH dependent modulation of gap junction networks.
Li A, Cho JH, Reid B, Tseng CC, He L, Tan P, Yeh CY, Wu P, Li Y, Widelitz RB, Zhou Y, Zhao M, Chow RH, Chuong CM
Nature communications 2018 Dec 18;9(1):5377
Nature communications 2018 Dec 18;9(1):5377
Technical Advance: New in vitro method for assaying the migration of primary B cells using an endothelial monolayer as substrate.
Stewart-Hutchinson PJ, Szasz TP, Jaeger ER, Onken MD, Cooper JA, Morley SC
Journal of leukocyte biology 2017 Sep;102(3):941-948
Journal of leukocyte biology 2017 Sep;102(3):941-948
Experimental identification and computational characterization of a novel extracellular metalloproteinase produced by Clostridium sordellii.
Aldape MJ, Tao A, Heeney DD, McIndoo ER, French JM, Xu D
RSC advances 2017 Mar 2;7(23):13928-13938
RSC advances 2017 Mar 2;7(23):13928-13938
Tandem CAR T cells targeting HER2 and IL13Rα2 mitigate tumor antigen escape.
Hegde M, Mukherjee M, Grada Z, Pignata A, Landi D, Navai SA, Wakefield A, Fousek K, Bielamowicz K, Chow KK, Brawley VS, Byrd TT, Krebs S, Gottschalk S, Wels WS, Baker ML, Dotti G, Mamonkin M, Brenner MK, Orange JS, Ahmed N
The Journal of clinical investigation 2016 Aug 1;126(8):3036-52
The Journal of clinical investigation 2016 Aug 1;126(8):3036-52
p38 MAP kinase inhibitor suppresses transforming growth factor-β2-induced type 1 collagen production in trabecular meshwork cells.
Inoue-Mochita M, Inoue T, Fujimoto T, Kameda T, Awai-Kasaoka N, Ohtsu N, Kimoto K, Tanihara H
PloS one 2015;10(3):e0120774
PloS one 2015;10(3):e0120774
West Nile virus-induced cell adhesion molecules on human brain microvascular endothelial cells regulate leukocyte adhesion and modulate permeability of the in vitro blood-brain barrier model.
Roe K, Orillo B, Verma S
PloS one 2014;9(7):e102598
PloS one 2014;9(7):e102598
Phenolic acid composition, antiatherogenic and anticancer potential of honeys derived from various regions in Greece.
Spilioti E, Jaakkola M, Tolonen T, Lipponen M, Virtanen V, Chinou I, Kassi E, Karabournioti S, Moutsatsou P
PloS one 2014;9(4):e94860
PloS one 2014;9(4):e94860
Uveal melanoma cells utilize a novel route for transendothelial migration.
Onken MD, Li J, Cooper JA
PloS one 2014;9(12):e115472
PloS one 2014;9(12):e115472
A composite model of the human postcapillary venule for investigation of microvascular leukocyte recruitment.
Lauridsen HM, Pober JS, Gonzalez AL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Mar;28(3):1166-80
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Mar;28(3):1166-80
Interaction of mesenchymal stem cells with fibroblast-like synoviocytes via cadherin-11 promotes angiogenesis by enhanced secretion of placental growth factor.
Park SJ, Kim KJ, Kim WU, Cho CS
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3003-10
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3003-10
GroEL1, a heat shock protein 60 of Chlamydia pneumoniae, impairs neovascularization by decreasing endothelial progenitor cell function.
Lin YW, Huang CY, Chen YH, Shih CM, Tsao NW, Lin CY, Chang NC, Tsai CS, Tsai HY, Tsai JC, Huang PH, Li CY, Lin FY
PloS one 2013;8(12):e84731
PloS one 2013;8(12):e84731
IL-15 expression on RA synovial fibroblasts promotes B cell survival.
Benito-Miguel M, García-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibáñez T, Bonilla-Hernán MG, Pérez de Ayala C, Sánchez-Mateos P, Martín-Mola E, Miranda-Carús ME
PloS one 2012;7(7):e40620
PloS one 2012;7(7):e40620
Macrophages induce differentiation of plasma cells through CXCL10/IP-10.
Xu W, Joo H, Clayton S, Dullaers M, Herve MC, Blankenship D, De La Morena MT, Balderas R, Picard C, Casanova JL, Pascual V, Oh S, Banchereau J
The Journal of experimental medicine 2012 Sep 24;209(10):1813-23, S1-2
The Journal of experimental medicine 2012 Sep 24;209(10):1813-23, S1-2
Adhesion of human haematopoietic (CD34+) stem cells to human liver compartments is integrin and CD44 dependent and modulated by CXCR3 and CXCR4.
Crosby HA, Lalor PF, Ross E, Newsome PN, Adams DH
Journal of hepatology 2009 Oct;51(4):734-49
Journal of hepatology 2009 Oct;51(4):734-49
Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies.
Ylöstalo J, Bazhanov N, Prockop DJ
Experimental hematology 2008 Oct;36(10):1390-402
Experimental hematology 2008 Oct;36(10):1390-402
Involvement of native TRPC3 proteins in ATP-dependent expression of VCAM-1 and monocyte adherence in coronary artery endothelial cells.
Smedlund K, Vazquez G
Arteriosclerosis, thrombosis, and vascular biology 2008 Nov;28(11):2049-55
Arteriosclerosis, thrombosis, and vascular biology 2008 Nov;28(11):2049-55
SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration.
Kelly KA, Allport JR, Yu AM, Sinh S, Sage EH, Gerszten RE, Weissleder R
Journal of leukocyte biology 2007 Mar;81(3):748-56
Journal of leukocyte biology 2007 Mar;81(3):748-56
Cannabidiol attenuates high glucose-induced endothelial cell inflammatory response and barrier disruption.
Rajesh M, Mukhopadhyay P, Bátkai S, Haskó G, Liaudet L, Drel VR, Obrosova IG, Pacher P
American journal of physiology. Heart and circulatory physiology 2007 Jul;293(1):H610-9
American journal of physiology. Heart and circulatory physiology 2007 Jul;293(1):H610-9
LIGHT up-regulated on B lymphocytes and monocytes in rheumatoid arthritis mediates cellular adhesion and metalloproteinase production by synoviocytes.
Kang YM, Kim SY, Kang JH, Han SW, Nam EJ, Kyung HS, Park JY, Kim IS
Arthritis and rheumatism 2007 Apr;56(4):1106-17
Arthritis and rheumatism 2007 Apr;56(4):1106-17
Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B
Blood 2007 Apr 1;109(7):2806-14
Blood 2007 Apr 1;109(7):2806-14
Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts.
Ramachandran R, Morice AH, Compton SJ
American journal of respiratory cell and molecular biology 2006 Jul;35(1):133-41
American journal of respiratory cell and molecular biology 2006 Jul;35(1):133-41
The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
Fitau J, Boulday G, Coulon F, Quillard T, Charreau B
The Journal of biological chemistry 2006 Jul 21;281(29):20148-59
The Journal of biological chemistry 2006 Jul 21;281(29):20148-59
CC chemokines and transmigration of eosinophils in the presence of vascular cell adhesion molecule 1.
Yamamoto H, Nagata M, Sakamoto Y
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 2005 Feb;94(2):292-300
Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology 2005 Feb;94(2):292-300
Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
Lawson C, Ainsworth M, Yacoub M, Rose M
Journal of immunology (Baltimore, Md. : 1950) 1999 Mar 1;162(5):2990-6
Journal of immunology (Baltimore, Md. : 1950) 1999 Mar 1;162(5):2990-6
Functions of domain 1 and 4 of vascular cell adhesion molecule-1 in alpha4 integrin-dependent adhesion under static and flow conditions are differentially regulated.
Abe Y, Ballantyne CM, Smith CW
Journal of immunology (Baltimore, Md. : 1950) 1996 Dec 1;157(11):5061-9
Journal of immunology (Baltimore, Md. : 1950) 1996 Dec 1;157(11):5061-9
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VCAM-1/CD106 in HUVEC Cells. VCAM-1/CD106 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Human VCAM-1/CD106 Monoclonal Antibody (Catalog # BBA5) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This antibody is not suitable for labeling formalin-fixed, paraffin-embedded tissue.
