MA1-16626

antibody from Invitrogen Antibodies

Targeting: VEGFA VEGF, VEGF-A, VPF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-16626

Provider

Invitrogen Antibodies

Product name

VEGF Monoclonal Antibody (VG76e)

Antibody type

Monoclonal

Antigen

Other

Description

VEGF-A is a hetero dimer, consisting of 2 chains of approximately 24kDa each.

Antibody clone number

VG76e

Concentration

1 mg/mL

References

Submitted references

Vascular injury of immature epiphyses impair stem cell engraftment in cartilage defects.

Rashidi A, Theruvath AJ, Huang CH, Wu W, Mahmoud EE, Jesu Raj JG, Marycz K, Daldrup-Link HE
Scientific reports 2022 Jul 9;12(1):11696

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VEGF in 30 µg human kidney protein. Samples were incubated in VEGF monoclonal antibody (Product # MA1-16626.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-VEGF Monoclonal Antibody (VG76e) (Product # MA1-16626) and 20 and 40 kDa bands corresponding to VEGF were observed. Recombinant VEGF protein (250 ng) was electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005). An uncharacterized band (*) was observed around 60 kDa.