MA5-14573

antibody from Invitrogen Antibodies

Targeting: VEGFA VEGF, VEGF-A, VPF

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14573

Provider

Invitrogen Antibodies

Product name

Anti-VEGF Monoclonal Antibody (SP28)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA5-14576 targets Alpha methylacyl-CoA-racemase in WB, IF and IHC (P) applications and shows reactivity with Human samples. The MA5-14576 immunogen is human p504s/AMACR polypeptide.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

SP28

Vial size

7 mL

Concentration

Lot Dependent

Storage

4° C

References

Submitted references

The role of bone marrow derived-mesenchymal stem cells in attenuation of kidney function in rats with diabetic nephropathy.

Abdel Aziz MT, Wassef MA, Ahmed HH, Rashed L, Mahfouz S, Aly MI, Hussein RE, Abdelaziz M
Diabetology & metabolic syndrome 2014 Mar 9;6(1):34

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Overexpression of MMP-1 and VEGF-C is associated with a less favorable prognosis in esophageal squamous cell carcinoma.

Tao YS, Ma XY, Chai DM, Ma L, Feng ZZ, Cheng ZN, Lai MD
Onkologie 2012;35(11):651-6

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Giant cell lesions of the jaws: does the level of vascularity and angiogenesis correlate with behavior?

Peacock ZS, Jordan RC, Schmidt BL
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons 2012 Aug;70(8):1860-6

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Anti-human vascular endothelial growth factor (VEGF) antibody selection for immunohistochemical staining of proliferating blood vessels.

van der Loos CM, Meijer-Jorna LB, Broekmans ME, Ploegmakers HP, Teeling P, de Boer OJ, van der Wal AC
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2010 Feb;58(2):109-18

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Local chronic hypoperfusion secondary to sinus high pressure seems to be mainly responsible for the formation of intracranial dural arteriovenous fistula.

Chen L, Mao Y, Zhou LF
Neurosurgery 2009 May;64(5):973-83; discussion 983

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Overexpression of phosphorylated mammalian target of rapamycin predicts lymph node metastasis and prognosis of chinese patients with gastric cancer.

Yu G, Wang J, Chen Y, Wang X, Pan J, Li G, Jia Z, Li Q, Yao JC, Xie K
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Mar 1;15(5):1821-9

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Organic dust-induced activation, adhesion to substrate and expression of intercellular adhesion molecules in THP-1 monocytes.

Burvall K, Palmberg L, Larsson K
Life sciences 2007 Apr 3;80(17):1598-607

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Expression of hypoxia-inducible factor-1 and vascular endothelial growth factor in response to venous hypertension.

Zhu Y, Lawton MT, Du R, Shwe Y, Chen Y, Shen F, Young WL, Yang GY
Neurosurgery 2006 Sep;59(3):687-96; discussion 687-96

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of VEGF was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with VEGF (SP28) Rabbit Monoclonal Antibody (Product # MA5-14573) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of VEGF was done on HUVEC-C cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with VEGF Rabbit Monoclonal Antibody (MA514573, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.