OPA1-10110

antibody from Invitrogen Antibodies

Targeting: VEGFA VEGF, VEGF-A, VPF

Antibody data

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Validation data

Product number: OPA1-10110 - Provider product page
Provider: Invitrogen Antibodies
Product name: VEGF Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: OPA1-10110 detects recombinant human VEGF. OPA1-10110 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~21 kDa protein representing recombinant human VEGF. OPA1-10110 immunogen is a synthetic peptide corresponding to residues A(27) P M A E G G G Q N H H E V V K F M D(45) of human VEGF.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 1 mL
Concentration: 0.2 mg/mL
Storage: 4° C, do not freeze

VEGFA protein structure - OPA1-10110 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Celastrol inhibits the angiogenesis of HG-induced hRECs. A The branching points in hRECs induced with HG and treated with different concentrations of celastrol were observed by tube formation assay. B Inverted micrographs of branching points in hRECs induced with HG and treated with different concentrations of celastrol. C Representative western blots and D quantitative analysis for expression of HIF-1alpha and VEGF in hRECs induced with HG and treated with different concentrations of celastrol was detected by Western blot analysis. E The mRNA expression of HIF-1alpha and VEGF in hRECs induced with HG and treated with different concentrations of celastrol was detected by RT-qPCR analysis. F The expression of ICAM-1 in hRECs induced with HG and treated with different concentrations of celastrol was detected by immunofluorescence assay. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs. NG group. # P < 0.05, ## P < 0.01 and ### P < 0.001 vs. HG group. P < 0.05 vs. HG + 0.5 muM cela group. $ P < 0.05 and $$$ P < 0.001 vs. HG + 1.0 muM cela group. Cela: celastrol

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 5 Celastrol inhibits the proliferation and invasion of HG-induced hRECs by down-regulating the HIF1alpha/VEGF signaling pathway. A The protein expression of HIF-1alpha and VEGF in HG-induced hRECs treated with DMOG and celastrol was detected by Western blot analysis. B The proliferation of HG-induced hRECs treated with DMOG and celastrol was observed by MTT assay. C The invasion of HG-induced hRECs treated with DMOG and celastrol was observed by Transwell assay. D Optical micrographs of invasion of HG-induced hRECs treated with DMOG and celastrol. * P < 0.05, ** P < 0.01 and *** P < 0.001 vs. NG group. ## P < 0.01 and ### P < 0.001 vs. HG group. P < 0.05 and P < 0.001 vs. HG + cela group. Cela: celastrol