101-M56

antibody from ReliaTech GmbH

Targeting: VEGFA VEGF, VEGF-A, VPF

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

101-M56

Provider

ReliaTech GmbH

Product name

VEGF-A

Antibody type

Monoclonal

Antigen

recombinant human VEGF165

Description

antibody purified from hybridoma supernatant

Reactivity

Human

Host

Mouse

Antigen sequence

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDI
FQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVP
TEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECR
PKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCK
NTDSRCKARQLELNERTCRCDKPRR


Isotype

IgG

Antibody clone number

(#3C5)

Vial size

100 µl

Storage

Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

Handling

Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.

References

Submitted references

Reduction of circulating soluble Flt-1 alleviates preeclampsia-like symptoms in a mouse model

Bergmann A, Ahmad S, Cudmore M, Gruber A, Wittschen P, Lindenmaier W, Christofori G, Gross V, Gonzalves A, Gröne H, Ahmed A, Weich H
Journal of Cellular and Molecular Medicine 2010 June;14(6b):1857-1867

Western blot

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Western blot analysis of human VEGF-A isoforms 121, 165 and 189 (all produced in E. coli) under reducing (left panel) and non-reducing conditions (right panel).

Sample type

Purified recombinant proteins

ELISA

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

VEGF-A Sandwich-ELISA using VEGF189 (Cat# 300-094) as standard. Mouse anti-human VEGF-A (Cat# 101-M56) was used as capture antibody, Biotinylated mouse anti-human VEGF-A #339 (Cat# 101-MBi60) was used for detection.