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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [22]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF564 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal VEGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects rat VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) VEGF165 and rhVEGF121 is observed and less than 2% cross-reactivity with rhVEGF-B, recombinant mouse (rm) VEGF-B, rhVEGF-C, rhVEGF-D, and rmVEGF-D is observed. In Western blots, detection of recombinant mouse VEGF165 is observed.
- Reactivity
- Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Cellular Mechanisms of Angiogenesis in Neonatal Rat Models of Retinal Neurodegeneration.
Myocardial infarction stabilization by cell-based expression of controlled Vascular Endothelial Growth Factor levels.
Bones in human CYP26B1 deficiency and rats with hypervitaminosis A phenocopy Vegfa overexpression.
The effect of a single anti-Vascular Endothelial Growth Factor injection on neonatal growth and organ development: In-vivo study.
Coronary angiogenic effect of long-term administration of Nigella sativa.
Genome-wide expression differences in anti-Vegf and dexamethasone treatment of inflammatory angiogenesis in the rat cornea.
FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy.
Renal primordia activate kidney regenerative events in a rat model of progressive renal disease.
Quantitative analyses of retinal vascular area and density after different methods to reduce VEGF in a rat model of retinopathy of prematurity.
The probiotic mixture VSL#3 accelerates gastric ulcer healing by stimulating vascular endothelial growth factor.
Long-term gene therapy with thrombospondin 2 inhibits TGF-β activation, inflammation and angiogenesis in chronic allograft nephropathy.
Anti-VEGF antibody leads to later atypical intravitreous neovascularization and activation of angiogenic pathways in a rat model of retinopathy of prematurity.
Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure.
Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor.
VEGF promotes vascular sympathetic innervation.
Neutralizing VEGF decreases tortuosity and alters endothelial cell division orientation in arterioles and veins in a rat model of ROP: relevance to plus disease.
Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1.
Enhancement of glucose transporter expression of brain endothelial cells by vascular endothelial growth factor derived from glioma exposed to hypoxia.
Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions.
Effects of red wine polyphenols on postischemic neovascularization model in rats: low doses are proangiogenic, high doses anti-angiogenic.
Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
Inhibition of hypoxia-induced increase of blood-brain barrier permeability by YC-1 through the antagonism of HIF-1alpha accumulation and VEGF expression.
Asano D, Hokazono M, Hirano S, Morita A, Nakahara T
International journal of molecular sciences 2019 Sep 25;20(19)
International journal of molecular sciences 2019 Sep 25;20(19)
Myocardial infarction stabilization by cell-based expression of controlled Vascular Endothelial Growth Factor levels.
Melly L, Cerino G, Frobert A, Cook S, Giraud MN, Carrel T, Tevaearai Stahel HT, Eckstein F, Rondelet B, Marsano A, Banfi A
Journal of cellular and molecular medicine 2018 May;22(5):2580-2591
Journal of cellular and molecular medicine 2018 May;22(5):2580-2591
Bones in human CYP26B1 deficiency and rats with hypervitaminosis A phenocopy Vegfa overexpression.
Lind T, Lugano R, Gustafson AM, Norgård M, van Haeringen A, Dimberg A, Melhus H, Robertson SP, Andersson G
Bone reports 2018 Dec;9:27-36
Bone reports 2018 Dec;9:27-36
The effect of a single anti-Vascular Endothelial Growth Factor injection on neonatal growth and organ development: In-vivo study.
Khalili S, Shifrin Y, Pan J, Belik J, Mireskandari K
Experimental eye research 2018 Apr;169:54-59
Experimental eye research 2018 Apr;169:54-59
Coronary angiogenic effect of long-term administration of Nigella sativa.
Al Asoom LI
BMC complementary and alternative medicine 2017 Jun 13;17(1):308
BMC complementary and alternative medicine 2017 Jun 13;17(1):308
Genome-wide expression differences in anti-Vegf and dexamethasone treatment of inflammatory angiogenesis in the rat cornea.
Mirabelli P, Mukwaya A, Lennikov A, Xeroudaki M, Peebo B, Schaupper M, Lagali N
Scientific reports 2017 Aug 15;7(1):7616
Scientific reports 2017 Aug 15;7(1):7616
FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy.
Deliyanti D, Zhang Y, Khong F, Berka DR, Stapleton DI, Kelly DJ, Wilkinson-Berka JL
PloS one 2015;10(7):e0134392
PloS one 2015;10(7):e0134392
Renal primordia activate kidney regenerative events in a rat model of progressive renal disease.
Imberti B, Corna D, Rizzo P, Xinaris C, Abbate M, Longaretti L, Cassis P, Benedetti V, Benigni A, Zoja C, Remuzzi G, Morigi M
PloS one 2015;10(3):e0120235
PloS one 2015;10(3):e0120235
Quantitative analyses of retinal vascular area and density after different methods to reduce VEGF in a rat model of retinopathy of prematurity.
Wang H, Yang Z, Jiang Y, Flannery J, Hammond S, Kafri T, Vemuri SK, Jones B, Hartnett ME
Investigative ophthalmology & visual science 2014 Feb 4;55(2):737-44
Investigative ophthalmology & visual science 2014 Feb 4;55(2):737-44
The probiotic mixture VSL#3 accelerates gastric ulcer healing by stimulating vascular endothelial growth factor.
Dharmani P, De Simone C, Chadee K
PloS one 2013;8(3):e58671
PloS one 2013;8(3):e58671
Long-term gene therapy with thrombospondin 2 inhibits TGF-β activation, inflammation and angiogenesis in chronic allograft nephropathy.
Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C
PloS one 2013;8(12):e83846
PloS one 2013;8(12):e83846
Anti-VEGF antibody leads to later atypical intravitreous neovascularization and activation of angiogenic pathways in a rat model of retinopathy of prematurity.
McCloskey M, Wang H, Jiang Y, Smith GW, Strange J, Hartnett ME
Investigative ophthalmology & visual science 2013 Mar 21;54(3):2020-6
Investigative ophthalmology & visual science 2013 Mar 21;54(3):2020-6
Vascular endothelial growth factor is crucial for erythropoietin-induced improvement of cardiac function in heart failure.
Westenbrink BD, Ruifrok WP, Voors AA, Tilton RG, van Veldhuisen DJ, Schoemaker RG, van Gilst WH, de Boer RA
Cardiovascular research 2010 Jul 1;87(1):30-9
Cardiovascular research 2010 Jul 1;87(1):30-9
Production of scFv antibody fragments from a hybridoma with functional activity against human vascular endothelial growth factor.
Irani Y, Brereton HM, Tilton RG, Coster DJ, Williams KA
Hybridoma (2005) 2009 Jun;28(3):205-9
Hybridoma (2005) 2009 Jun;28(3):205-9
VEGF promotes vascular sympathetic innervation.
Marko SB, Damon DH
American journal of physiology. Heart and circulatory physiology 2008 Jun;294(6):H2646-52
American journal of physiology. Heart and circulatory physiology 2008 Jun;294(6):H2646-52
Neutralizing VEGF decreases tortuosity and alters endothelial cell division orientation in arterioles and veins in a rat model of ROP: relevance to plus disease.
Hartnett ME, Martiniuk D, Byfield G, Geisen P, Zeng G, Bautch VL
Investigative ophthalmology & visual science 2008 Jul;49(7):3107-14
Investigative ophthalmology & visual science 2008 Jul;49(7):3107-14
Heme oxygenase-1 promotes neovascularization in ischemic heart by coinduction of VEGF and SDF-1.
Lin HH, Chen YH, Chang PF, Lee YT, Yet SF, Chau LY
Journal of molecular and cellular cardiology 2008 Jul;45(1):44-55
Journal of molecular and cellular cardiology 2008 Jul;45(1):44-55
Enhancement of glucose transporter expression of brain endothelial cells by vascular endothelial growth factor derived from glioma exposed to hypoxia.
Yeh WL, Lin CJ, Fu WM
Molecular pharmacology 2008 Jan;73(1):170-7
Molecular pharmacology 2008 Jan;73(1):170-7
Role of VEGF in maintaining renal structure and function under normotensive and hypertensive conditions.
Advani A, Kelly DJ, Advani SL, Cox AJ, Thai K, Zhang Y, White KE, Gow RM, Marshall SM, Steer BM, Marsden PA, Rakoczy PE, Gilbert RE
Proceedings of the National Academy of Sciences of the United States of America 2007 Sep 4;104(36):14448-53
Proceedings of the National Academy of Sciences of the United States of America 2007 Sep 4;104(36):14448-53
Effects of red wine polyphenols on postischemic neovascularization model in rats: low doses are proangiogenic, high doses anti-angiogenic.
Baron-Menguy C, Bocquet A, Guihot AL, Chappard D, Amiot MJ, Andriantsitohaina R, Loufrani L, Henrion D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Nov;21(13):3511-21
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Nov;21(13):3511-21
Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy.
Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T
American journal of physiology. Heart and circulatory physiology 2007 Jul;293(1):H474-81
American journal of physiology. Heart and circulatory physiology 2007 Jul;293(1):H474-81
Inhibition of hypoxia-induced increase of blood-brain barrier permeability by YC-1 through the antagonism of HIF-1alpha accumulation and VEGF expression.
Yeh WL, Lu DY, Lin CJ, Liou HC, Fu WM
Molecular pharmacology 2007 Aug;72(2):440-9
Molecular pharmacology 2007 Aug;72(2):440-9
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Rat and Mouse VEGF by Western Blot. Detection of Recombinant Rat and Mouse VEGF by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- VEGF164in Rat Kidney. VEGF164in Rat Kidney.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by VEGF164and Neutralization by Rat VEGF Antibody. Cell Proliferation Induced by VEGF164and Neutralization by Rat VEGF Antibody.
