Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA303731 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit monoclonal antibody against VEGF (C-term)(EP1176Y)
- Antibody type
- Monoclonal
- Description
- Rabbit monoclonal antibody against VEGF (C-term)(EP1176Y)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- VEGFA
- Isotype
- IgG
- Antibody clone number
- EP1176Y
- Vial size
- 100 µl
- Concentration
- NULL
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/ Immunofluorescence - VEGF antibody [EP1176Y]; Immunofluorescent staining of HUVEC cells using TA303731 (1:250).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IHC - Anti-VEGF antibody; TA303731 stainingVEGF in Mouse kidney tissue sections by IHC (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100and blocked with 5% serum for45 minutes at 25°C. Samples were incubated with primary antibody (1/400 in4% BSA + 5% serum in PBST) for 14 hours at 4°C. An Alexa Fluor? 546-conjugated Donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin-embedded sections) - VEGF antibody [EP1176Y]; Ab52917 (1:50) staining human VEGF in human hemangioma tissue by immunohistochemistry using paraffin embedded tissue.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry - VEGF antibody- Carboxyterminal end; Overlay histogram showing HeLa cells stained with TA303731 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells ) used under the same conditions. This antibody gave a positive signal in HeLa cells fixed with 80% methanol/permeabilized in 0.1% PBS-Tween used under the same conditions.
- Validation comment
- FC
