Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB293-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal VEGF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human VEGF165 and human VEGF121 in ELISAs and Western blots. In ELISAs, approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and recombinant rat (rr)VEGF and no cross-reactivity with recombinant human (rh) VEGF-D is obseved.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Recombinant Human VEGF by Western Blot. Detection of Recombinant Human VEGF by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate VEGF Monoclonal Antibody (Catalog # MAB293). The ND50 is typically 10-60 ng/mL.