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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB1603 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine VEGF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects canine VEGF in ELISAs and Western blots. In sandwich immunoassays, 100% cross-reactivity with recombinant human (rh) VEGF121 and rhVEGF165, less than 25% cross-reactivity with recombinant rat VEGF164, and no cross-reactivity with rhVEGF-B186, rhVEGF-C, rhVEGF-D, recombinant mouse (rm) VEGF115, rmVEGF120, rmVEGF165, or recombinant zebrafish VEGF165 is observed.
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NP_001103972- Isotype
- IgG
- Antibody clone number
- 247109
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A functional comparison of canine and murine bone marrow derived cultured mast cells.
Lin TY, London CA
Veterinary immunology and immunopathology 2006 Dec 15;114(3-4):320-34
Veterinary immunology and immunopathology 2006 Dec 15;114(3-4):320-34
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- VEGF in Canine PBMCs. VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Canine VEGF Monoclonal Antibody (Catalog # MAB1603) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
