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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB1247 - Provider product page
- Provider
- R&D Systems
- Product name
- Zebrafish VEGF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects zebrafish VEGF in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) VEGF121, 165, 206, rhVEGF-B186, rhVEGF-C, rhVEGF-D, recombinant mouse (rm) VEGF115, rmVEGF165, rmVEGF-B186, rmVEGF-D, or recombinant rat VEGF164 is observed.
- Reactivity
- Zebrafish
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
O73682.1- Isotype
- IgG
- Antibody clone number
- 211615
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CUL2 is required for the activity of hypoxia-inducible factor and vasculogenesis.
Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: possible insights from emerging technology.
Maeda Y, Suzuki T, Pan X, Chen G, Pan S, Bartman T, Whitsett JA
The Journal of biological chemistry 2008 Jun 6;283(23):16084-92
The Journal of biological chemistry 2008 Jun 6;283(23):16084-92
Microarray gene expression profiling of cell lines from primary and metastatic tongue squamous cell carcinoma: possible insights from emerging technology.
Vigneswaran N, Wu J, Sacks P, Gilcrease M, Zacharias W
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2005 Feb;34(2):77-86
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 2005 Feb;34(2):77-86
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by VEGF165 and Neutralization by Zebrafish VEGF Antibody. Recombinant Zebrafish VEGF165 (Catalog # 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF165 (100 ng/mL) is neutrallized (green line) by increasing concentrations of Mouse Anti-Zebrafish VEGF Monoclonal Antibody (Catalog # MAB1247). The ND50 is typically 0.2-0.5 µg/mL.
