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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF1603 - Provider product page
- Provider
- R&D Systems
- Product name
- Canine VEGF164 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects canine VEGF164 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human VEGF, recombinant mouse VEGF, and recombinant rat VEGF is observed.
- Reactivity
- Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9MYV3- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Evaluation of a xenogeneic VEGF vaccine in dogs with soft tissue sarcoma.
Kamstock D, Elmslie R, Thamm D, Dow S
Cancer immunology, immunotherapy : CII 2007 Aug;56(8):1299-309
Cancer immunology, immunotherapy : CII 2007 Aug;56(8):1299-309
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VEGF in Canine PBMCs. VEGF was detected in immersion fixed canine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Canine VEGF 164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by VEGF and Neutralization by Canine VEGF Antibody. Recombinant Canine VEGF (Catalog # 1603-CV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine VEGF (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1603). The ND50 is typically 0.04-0.10 µg/mL.
