AF1247

antibody from R&D Systems

Targeting: VEGFA VEGF, VEGF-A, VPF

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1247

Provider

R&D Systems

Product name

Zebrafish VEGF Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects zebrafish VEGF in direct ELISAs and Western blots. In Western blots, less than 2% cross-reactivity with recombinant human VEGF165, recombinant mouse VEGF164, and recombinant rat VEGF164 (under non-reducing conditions) is observed.

Reactivity

Zebrafish

Host

Goat

Conjugate

Unconjugated

Antigen sequence

O73682


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by VEGF₁₆₅ and Neutralization by Zebrafish VEGF Antibody. Recombinant Zebrafish VEGF₁₆₅ (Catalog # 1247-ZV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Zebrafish VEGF₁₆₅ (80 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Zebrafish VEGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1247). The ND₅₀ is typically 0.3-0.6 µg/mL.