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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- R31500 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- VEGF Antibody / VEGFA
- Antibody type
- Polyclonal
- Description
- This highly specific VEGF antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human and rat samples.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the VEGF antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of VEGF antibody (A isoform) and Lane 1: rat thymus; 2: rat brain lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) 20ng/lane recombinant protein, 2) 10ng/lane recombinant protein, 3) human U-87 MG, 4) human K562, 5) human U-251, 6) rat brain, 7) rat C6, 8) mouse brain and 9) mouse Neuro-2a cell lysate with VEGF antibody. Predicted molecular weight 19~22 kDa (monomer) and 38~44 kDa (dimer).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: VEGF antibody testing of human placenta tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: VEGF antibody testing of human lung cancer tissue
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human ovarian serous adenocarcinoma tissue with VEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human lung cancer tissue with VEGF antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed human K562 cells with VEGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VEGF antibody.
