MA5-23719

antibody from Invitrogen Antibodies

Targeting: VEGFA VEGF, VEGF-A, VPF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23719

Provider

Invitrogen Antibodies

Product name

VEGF Monoclonal Antibody (26503)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant mouse (rm) VEGF and rrVEGF and no cross-reactivity with rhVEGF-D.

Antibody clone number

26503

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-VEGF Monoclonal Antibody (26503) (Product # MA5-23719) and 20 and 40 kDa bands corresponding to VEGF were observed. Recombinant VEGF protein (250 ng in Lane 1 and 25 ng in Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005). An uncharacterized band (*) was observed around 60 kDa.