P931

antibody from Invitrogen Antibodies

Targeting: VEGFA VEGF, VEGF-A, VPF

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

P931

Provider

Invitrogen Antibodies

Product name

Anti-VEGF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

PA1-002 has been successfully used in ELISA, Western Blot, immunofluorescence, and immunohistochemistry applications with human and mouse samples. Neoepitope antibodies distinguish smaller cleaved fragments or processed forms of proteins versus the intact full-length or precursor by using a designed peptide purification process to maximize immunoreactivity to a specific cleavage site. Human HTT caspase cleavage sites generate fragment-specific forms of the protein. Caspase-3/7 has been shown to generate cleavage sites at animo acids 513 and 552. Caspase-2 cleaves at amino acid 552 and caspase-6 at amino acid 586. Neo-specific antibody PA1-002 recognizes the 513 cleaved fragment without detecting the full-length form.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

500 ug

Concentration

1 mg/ml

Storage

-20°C

References

Submitted references

Significant improvement of direct reprogramming efficacy of fibroblasts into progenitor endothelial cells by ETV2 and hypoxia.

Van Pham P, Vu NB, Nguyen HT, Huynh OT, Truong MT
Stem cell research & therapy 2016 Aug 4;7(1):104

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Vascular Endothelial Growth Factor (VEGF, green) in Hela cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were stained with (left panel) or without (right panel) a VEGF polyclonal antibody (Product # P931) at a dilution of 1:40 for at least 1 hour at room temperature, and then incubated with a Dylight 488 goat anti-rabbit IgG secondary antibody at a dilution of 1:1000 for 45 minutes at room temperature. F-actin (both panels, red) was stained by Dylight 554 Phalloidin (Product # 21834) and nuclei (both panels, blue) were stained with DAPI (Product # 46190). Images were taken at 60X magnification.