NB600-548

antibody from Novus Biologicals

Targeting: VEGFA VEGF, VEGF-A, VPF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

NB600-548

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-548, RRID:AB_10002448

Product name

Mouse Monoclonal VEGF Antibody

Antibody type

Monoclonal

Description

Protein G purified. VEGF (5C3

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

500 uL

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

In vitro and in vivo evaluation of N,N,N-trimethylphytosphingosine-iodide (TMP) in liposomes for the treatment of angiogenesis and metastasis.

Song CK, Lee JH, Jahn A, Choi MJ, Namgoong SK, Hong SS, Chong S, Shim CK, Chung SJ, Kim DD
International journal of pharmaceutics 2012 Sep 15;434(1-2):191-8

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Analysis of mTOR inhibition-involved pathway in ovarian clear cell adenocarcinoma.

Harasawa M, Yasuda M, Hirasawa T, Miyazawa M, Shida M, Muramatsu T, Douguchi K, Matsui N, Takekoshi S, Kajiwara H, Yoshiyuki Osamura R, Mikami M
Acta histochemica et cytochemica 2011 Apr 28;44(2):113-8

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: VEGF Antibody (5C3.F8) [NB600-548] - Analysis of VEGF from Human Cells in a reducing gel.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: VEGF Antibody (5C3.F8) [NB600-548] - Analysis of 1ug of indicated recombinant VEGF isoforms, and 10ul of PageRuler PlusPrestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: VEGF Antibody (5C3.F8) [NB600-548] - Analysis of Vascular Endothelial Growth Factor (VEGF, green) in Hela cells. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% Blocker BSA for 30 minutes at room temperature. Cells were stained with (left panel) or without (right panel) a VEGF polyclonal antibody at a dilution of 1:20 for at least 1 hour at room temperature, and then incubated with a Dylight 488 goat anti-mouse IgG secondary antibody at a dilution of 1:1000 for 45 minutes at room temperature. F-actin (both panels, red) was stained by Dylight 554 Phalloidin and nuclei (both panels, blue) were stained with DAPI.