Antibody data
- Antibody Data
- Antigen structure
- References [41]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-293-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human VEGF165 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human VEGF in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant canine VEGF is observed, and less than 20% cross-reactivity with recombinant mouse VEGF165 and recombinant rat VEGF164 is observed, and less than 5% cross-reactivity with recombinant zebrafish VEGF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_001165097.1
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Rapid Production of Human VEGF-A following Intradermal Injection of Modified VEGF-A mRNA Demonstrated by Cutaneous Microdialysis in the Rabbit and Pig In Vivo.
Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages.
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.
Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease.
CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells.
Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression.
A randomized, single-blind, Phase I trial (INVICTAN-1) assessing the bioequivalence and safety of BI 695502, a bevacizumab biosimilar candidate, in healthy subjects.
PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently.
Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
Detection of VEGF-A(xxx)b isoforms in human tissues.
Simple patterned nanofiber scaffolds and its enhanced performance in immunoassay.
Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Chronic exposure of colorectal cancer cells to bevacizumab promotes compensatory pathways that mediate tumour cell migration.
VEGF in the lung: a role for novel isoforms.
Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo.
The hypoxia-inducible factor 1/NOR-1 axis regulates the survival response of endothelial cells to hypoxia.
Effect of chemotherapeutic stress on induction of vascular endothelial growth factor family members and receptors in human colorectal cancer cells.
Comparison of bevacizumab, ranibizumab, and pegaptanib in vitro: efficiency and possible additional pathways.
Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
Mammary alveolar development during lactation is inhibited by the endogenous antiangiogenic growth factor isoform, VEGF165b.
Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment.
Bactericidal/permeability-increasing protein's signaling pathways and its retinal trophic and anti-angiogenic effects.
The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
Inhibition of cyclooxygenase-2 disrupts tumor vascular mural cell recruitment and survival signaling.
Human peripheral blood eosinophils induce angiogenesis.
Age-associated aortic stenosis in apolipoprotein E-deficient mice.
The proline-rich homeodomain (PRH/HEX) protein is down-regulated in liver during infection with lymphocytic choriomeningitis virus.
Estrogen enhances angiogenesis through a pathway involving platelet-activating factor-mediated nuclear factor-kappaB activation.
Alterations in angiogenic growth factors and neuronal nitric oxide synthase expression in chronic cavernosal ischemia.
Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath.
Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.
Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
VEGF signaling is required for the assembly but not the maintenance of embryonic blood vessels.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Pehrsson S, Hölttä M, Linhardt G, Danielson RF, Carlsson L
BioMed research international 2019;2019:3915851
BioMed research international 2019;2019:3915851
Expression of ID4 protein in breast cancer cells induces reprogramming of tumour-associated macrophages.
Donzelli S, Milano E, Pruszko M, Sacconi A, Masciarelli S, Iosue I, Melucci E, Gallo E, Terrenato I, Mottolese M, Zylicz M, Zylicz A, Fazi F, Blandino G, Fontemaggi G
Breast cancer research : BCR 2018 Jun 19;20(1):59
Breast cancer research : BCR 2018 Jun 19;20(1):59
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.
Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
Reduced microRNA-503 expression augments lung fibroblast VEGF production in chronic obstructive pulmonary disease.
Ikari J, Nelson AJ, Obaid J, Giron-Martinez A, Ikari K, Makino F, Iwasawa S, Gunji Y, Farid M, Wang X, Basma H, Demeo D, Feghali-Bostwick C, Holz O, Rabe K, Liu X, Rennard SI
PloS one 2017;12(9):e0184039
PloS one 2017;12(9):e0184039
CD147 promotes the proliferation, invasiveness, migration and angiogenesis of human lung carcinoma cells.
Yang S, Qi F, Tang C, Wang H, Qin H, Li X, Li J, Wang W, Zhao C, Gao H
Oncology letters 2017 Feb;13(2):898-904
Oncology letters 2017 Feb;13(2):898-904
Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression.
Xu WW, Li B, Guan XY, Chung SK, Wang Y, Yip YL, Law SY, Chan KT, Lee NP, Chan KW, Xu LY, Li EM, Tsao SW, He QY, Cheung AL
Nature communications 2017 Feb 10;8:14399
Nature communications 2017 Feb 10;8:14399
A randomized, single-blind, Phase I trial (INVICTAN-1) assessing the bioequivalence and safety of BI 695502, a bevacizumab biosimilar candidate, in healthy subjects.
Hettema W, Wynne C, Lang B, Altendorfer M, Czeloth N, Lohmann R, Athalye S, Schliephake D
Expert opinion on investigational drugs 2017 Aug;26(8):889-896
Expert opinion on investigational drugs 2017 Aug;26(8):889-896
PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently.
Zheng L, Zhao C, Du Y, Lin X, Jiang Y, Lee C, Tian G, Mi J, Li X, Chen Q, Ye Z, Huang L, Wang S, Ren X, Xing L, Chen W, Huang D, Gao Z, Zhang S, Lu W, Tang Z, Wang B, Ju R, Li X
Oncotarget 2016 Nov 22;7(47):77902-77915
Oncotarget 2016 Nov 22;7(47):77902-77915
Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma.
Gao Y, Zhou Z, Lu S, Huang X, Zhang C, Jiang R, Yao A, Sun B, Wang X
Stem cells (Dayton, Ohio) 2016 Apr;34(4):1112-22
Stem cells (Dayton, Ohio) 2016 Apr;34(4):1112-22
Sustained delivery of VEGF maintains innervation and promotes reperfusion in ischemic skeletal muscles via NGF/GDNF signaling.
Shvartsman D, Storrie-White H, Lee K, Kearney C, Brudno Y, Ho N, Cezar C, McCann C, Anderson E, Koullias J, Tapia JC, Vandenburgh H, Lichtman JW, Mooney DJ
Molecular therapy : the journal of the American Society of Gene Therapy 2014 Jul;22(7):1243-1253
Molecular therapy : the journal of the American Society of Gene Therapy 2014 Jul;22(7):1243-1253
Endothelial cell adhesion to soluble vascular endothelial growth factor receptor-1 triggers a cell dynamic and angiogenic phenotype.
Orecchia A, Mettouchi A, Uva P, Simon GC, Arcelli D, Avitabile S, Ragone G, Meneguzzi G, Pfenninger KH, Zambruno G, Failla CM
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Feb;28(2):692-704
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Feb;28(2):692-704
Detection of VEGF-A(xxx)b isoforms in human tissues.
Bates DO, Mavrou A, Qiu Y, Carter JG, Hamdollah-Zadeh M, Barratt S, Gammons MV, Millar AB, Salmon AH, Oltean S, Harper SJ
PloS one 2013;8(7):e68399
PloS one 2013;8(7):e68399
Simple patterned nanofiber scaffolds and its enhanced performance in immunoassay.
Wang J, Kang QS, Lv XG, Song J, Zhan N, Dong WG, Huang WH
PloS one 2013;8(12):e82888
PloS one 2013;8(12):e82888
Platelet-derived growth factor C and calpain-3 are modulators of human melanoma cell invasiveness.
Ruffini F, Tentori L, Dorio AS, Arcelli D, D'Amati G, D'Atri S, Graziani G, Lacal PM
Oncology reports 2013 Dec;30(6):2887-96
Oncology reports 2013 Dec;30(6):2887-96
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Chronic exposure of colorectal cancer cells to bevacizumab promotes compensatory pathways that mediate tumour cell migration.
Fan F, Samuel S, Gaur P, Lu J, Dallas NA, Xia L, Bose D, Ramachandran V, Ellis LM
British journal of cancer 2011 Apr 12;104(8):1270-7
British journal of cancer 2011 Apr 12;104(8):1270-7
VEGF in the lung: a role for novel isoforms.
Varet J, Douglas SK, Gilmartin L, Medford AR, Bates DO, Harper SJ, Millar AB
American journal of physiology. Lung cellular and molecular physiology 2010 Jun;298(6):L768-74
American journal of physiology. Lung cellular and molecular physiology 2010 Jun;298(6):L768-74
Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
Angiogenesis 2010 Dec;13(4):337-47
Angiogenesis 2010 Dec;13(4):337-47
VEGF(121)b, a new member of the VEGF(xxx)b family of VEGF-A splice isoforms, inhibits neovascularisation and tumour growth in vivo.
Rennel ES, Varey AH, Churchill AJ, Wheatley ER, Stewart L, Mather S, Bates DO, Harper SJ
British journal of cancer 2009 Oct 6;101(7):1183-93
British journal of cancer 2009 Oct 6;101(7):1183-93
The hypoxia-inducible factor 1/NOR-1 axis regulates the survival response of endothelial cells to hypoxia.
Martorell L, Gentile M, Rius J, Rodríguez C, Crespo J, Badimon L, Martínez-González J
Molecular and cellular biology 2009 Nov;29(21):5828-42
Molecular and cellular biology 2009 Nov;29(21):5828-42
Effect of chemotherapeutic stress on induction of vascular endothelial growth factor family members and receptors in human colorectal cancer cells.
Fan F, Gray MJ, Dallas NA, Yang AD, Van Buren G 2nd, Camp ER, Ellis LM
Molecular cancer therapeutics 2008 Sep;7(9):3064-70
Molecular cancer therapeutics 2008 Sep;7(9):3064-70
Comparison of bevacizumab, ranibizumab, and pegaptanib in vitro: efficiency and possible additional pathways.
Klettner A, Roider J
Investigative ophthalmology & visual science 2008 Oct;49(10):4523-7
Investigative ophthalmology & visual science 2008 Oct;49(10):4523-7
Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
The Journal of biological chemistry 2008 Dec 5;283(49):34250-9
The Journal of biological chemistry 2008 Dec 5;283(49):34250-9
Mammary alveolar development during lactation is inhibited by the endogenous antiangiogenic growth factor isoform, VEGF165b.
Qiu Y, Bevan H, Weeraperuma S, Wratting D, Murphy D, Neal CR, Bates DO, Harper SJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Apr;22(4):1104-12
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Apr;22(4):1104-12
Vascular endothelial growth factor-A induces plaque expansion in ApoE knock-out mice by promoting de novo leukocyte recruitment.
Lucerna M, Zernecke A, de Nooijer R, de Jager SC, Bot I, van der Lans C, Kholova I, Liehn EA, van Berkel TJ, Yla-Herttuala S, Weber C, Biessen EA
Blood 2007 Jan 1;109(1):122-9
Blood 2007 Jan 1;109(1):122-9
Bactericidal/permeability-increasing protein's signaling pathways and its retinal trophic and anti-angiogenic effects.
Yamagata M, Rook SL, Sassa Y, Ma RC, Geraldes P, Goddard L, Clermont A, Gao B, Salti H, Gundel R, White M, Feener EP, Aiello LP, King GL
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Oct;20(12):2058-67
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2006 Oct;20(12):2058-67
The endogenous anti-angiogenic family of splice variants of VEGF, VEGFxxxb, are down-regulated in pre-eclamptic placentae at term.
Bates DO, MacMillan PP, Manjaly JG, Qiu Y, Hudson SJ, Bevan HS, Hunter AJ, Soothill PW, Read M, Donaldson LF, Harper SJ
Clinical science (London, England : 1979) 2006 May;110(5):575-85
Clinical science (London, England : 1979) 2006 May;110(5):575-85
Insulin-like growth factor binding protein-4 (IGFBP-4) is a novel anti-angiogenic and anti-tumorigenic mediator secreted by dibutyryl cyclic AMP (dB-cAMP)-differentiated glioblastoma cells.
Moreno MJ, Ball M, Andrade MF, McDermid A, Stanimirovic DB
Glia 2006 Jun;53(8):845-57
Glia 2006 Jun;53(8):845-57
Inhibition of cyclooxygenase-2 disrupts tumor vascular mural cell recruitment and survival signaling.
Lee A, Frischer J, Serur A, Huang J, Bae JO, Kornfield ZN, Eljuga L, Shawber CJ, Feirt N, Mansukhani M, Stempak D, Baruchel S, Glade Bender J, Kandel JJ, Yamashiro DJ
Cancer research 2006 Apr 15;66(8):4378-84
Cancer research 2006 Apr 15;66(8):4378-84
Human peripheral blood eosinophils induce angiogenesis.
Puxeddu I, Alian A, Piliponsky AM, Ribatti D, Panet A, Levi-Schaffer F
The international journal of biochemistry & cell biology 2005 Mar;37(3):628-36
The international journal of biochemistry & cell biology 2005 Mar;37(3):628-36
Age-associated aortic stenosis in apolipoprotein E-deficient mice.
Tanaka K, Sata M, Fukuda D, Suematsu Y, Motomura N, Takamoto S, Hirata Y, Nagai R
Journal of the American College of Cardiology 2005 Jul 5;46(1):134-41
Journal of the American College of Cardiology 2005 Jul 5;46(1):134-41
The proline-rich homeodomain (PRH/HEX) protein is down-regulated in liver during infection with lymphocytic choriomeningitis virus.
Djavani M, Topisirovic I, Zapata JC, Sadowska M, Yang Y, Rodas J, Lukashevich IS, Bogue CW, Pauza CD, Borden KL, Salvato MS
Journal of virology 2005 Feb;79(4):2461-73
Journal of virology 2005 Feb;79(4):2461-73
Estrogen enhances angiogenesis through a pathway involving platelet-activating factor-mediated nuclear factor-kappaB activation.
Seo KH, Lee HS, Jung B, Ko HM, Choi JH, Park SJ, Choi IH, Lee HK, Im SY
Cancer research 2004 Sep 15;64(18):6482-8
Cancer research 2004 Sep 15;64(18):6482-8
Alterations in angiogenic growth factors and neuronal nitric oxide synthase expression in chronic cavernosal ischemia.
Wang T, Soker S, Atala A, Siroky MB, Azadzoi KM
International journal of impotence research 2004 Oct;16(5):403-11
International journal of impotence research 2004 Oct;16(5):403-11
Long-term reduction of medial and intimal thickening in porcine saphenous vein grafts with a polyglactin biodegradable external sheath.
Vijayan V, Shukla N, Johnson JL, Gadsdon P, Angelini GD, Smith FC, Baird R, Jeremy JY
Journal of vascular surgery 2004 Nov;40(5):1011-9
Journal of vascular surgery 2004 Nov;40(5):1011-9
Cigarette smoke induces persisting increases of vasoactive mediators in pulmonary arteries.
Wright JL, Tai H, Churg A
American journal of respiratory cell and molecular biology 2004 Nov;31(5):501-9
American journal of respiratory cell and molecular biology 2004 Nov;31(5):501-9
Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
International journal of cancer 2003 May 1;104(5):603-10
International journal of cancer 2003 May 1;104(5):603-10
Proangiogenic properties of human myeloma cells: production of angiopoietin-1 and its potential relationship to myeloma-induced angiogenesis.
Giuliani N, Colla S, Lazzaretti M, Sala R, Roti G, Mancini C, Bonomini S, Lunghi P, Hojden M, Genestreti G, Svaldi M, Coser P, Fattori PP, Sammarelli G, Gazzola GC, Bataille R, Almici C, Caramatti C, Mangoni L, Rizzoli V
Blood 2003 Jul 15;102(2):638-45
Blood 2003 Jul 15;102(2):638-45
VEGF signaling is required for the assembly but not the maintenance of embryonic blood vessels.
Argraves WS, Larue AC, Fleming PA, Drake CJ
Developmental dynamics : an official publication of the American Association of Anatomists 2002 Nov;225(3):298-304
Developmental dynamics : an official publication of the American Association of Anatomists 2002 Nov;225(3):298-304
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human VEGF by Western Blot. Western blot shows lysates of human lung tissue, human kidney tissue, and human liver tissue. PVDF membrane was probed with 5-10 µg/mL of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for VEGF at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VEGF in HUVEC Cells. VEGF was detected in immersion fixed human umbilical vein endothelial cells (HUVECs) using Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VEGF in Human Liver Cancer Tissue. VEGF was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Goat Anti-Human VEGF 165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by VEGF165 and Neutralization by Human VEGF Antibody. Recombinant Human VEGF165 (Catalog # 293-VE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human VEGF165 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human VEGF165 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-293-NA). The ND50 is typically 0.02-0.12 µg/mL.