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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- IC2931A - Provider product page
- Provider
- R&D Systems
- Product name
- Human VEGF APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human VEGF.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- 23410
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references TGFb1 suppresses the activation of distinct dNK subpopulations in preeclampsia.
Effects of the vascular endothelial growth factor receptor-2 (VEGFR-2) inhibitor SU5416 on in vitro cultures of Plasmodium falciparum.
Nicotinamide inhibits vasculogenic mimicry, an alternative vascularization pathway observed in highly aggressive melanoma.
Zhang J, Dunk CE, Shynlova O, Caniggia I, Lye SJ
EBioMedicine 2019 Jan;39:531-539
EBioMedicine 2019 Jan;39:531-539
Effects of the vascular endothelial growth factor receptor-2 (VEGFR-2) inhibitor SU5416 on in vitro cultures of Plasmodium falciparum.
Hempel C, Hoyer N, Staalsø T, Kurtzhals JA
Malaria journal 2014 May 28;13:201
Malaria journal 2014 May 28;13:201
Nicotinamide inhibits vasculogenic mimicry, an alternative vascularization pathway observed in highly aggressive melanoma.
Itzhaki O, Greenberg E, Shalmon B, Kubi A, Treves AJ, Shapira-Frommer R, Avivi C, Ortenberg R, Ben-Ami E, Schachter J, Besser MJ, Markel G
PloS one 2013;8(2):e57160
PloS one 2013;8(2):e57160
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of VEGF in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line either treated with 50 ng/mL PMA and 200 ng/mLCalcium Ionomycin for 16 hours (filled histogram) or untreated (open histogram) was stained with Mouse Anti-Human VEGF APC-conjugated Monoclonal Antibody (Catalog # IC2931A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
