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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- GTX60359 - Provider product page
- Provider
- GeneTex
- Product name
- MBD1 antibody - ChIP grade
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- WB analysis of nuclear extracts (40 £gg) of HeLa cells using MBD1 antibody diluted 1:500 in TBS-Tween containing 5% skimmed milk.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- ELISA was performed using a serial dilution of MBD1 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:20,000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ChIP was performed with U2OS nuclear extract and 1.5 £gg of MBD1 antibody. Beads only were used as a negative IP control. The precipitated DNA was detected by QPCR with primer set targeting to GAPDH promoter or myoglobin.
