TA347239

antibody from OriGene

Targeting: MBD1 CXXC3, PCM1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

TA347239

Provider

OriGene

Product name

Rabbit Polyclonal MBD1 Antibody

Antibody type

Polyclonal

Description

Rabbit Polyclonal MBD1 Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

MBD1

Isotype

IgG

Antibody clone number

NULL

Vial size

50 µg

Concentration

0.7 ug/ul

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB using the antibody against MBD1 diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right, the marker (in kDa) is shown on the left.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human MBD1, crude serum and Flow Through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the purified antibody was estimated to be 1:20,000.

Validation comment

ELISA

Other assay

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

ChIP assays were performed using human osteosarcoma ( U2OS) cells, the ab against MBD1 and optimized PCR primer sets. Sheared chromatin from 1x10e6 cells and 1.5 ug of antibody were used per ChIP experiment. Beads only were negative IP control. qPCR was performed with primers for the promoters of the MLH1 gene (positive control) and CDC6 gene (negative control). Image shows the recovery, expressed as a % of input (the relative amount of IP'd DNA compared to input DNA after qPCR analysis).

Validation comment

Assay