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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14113 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14113, RRID:AB_10554138
- Product name
- MBD1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MBD1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Human osteosarcoma cells (U-2 OS) were transfected with an expression vector for TY1-tagged MBD1.The presence of the TY1-MBD1 in the cell lysates was demonstrated by western blot analysis with the antibody directed against the TY1-tag and with MBD1 polyclonal antibody (Cat # PAB14113) (lane 2), diluted 1 : 2,000 in TBST containing 3% milk powder.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of MBD1 polyclonal antibody (Cat # PAB14113), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution , the titer of the purified antibody was estimated to be 1 : 26,000.
- Validation comment
- ELISA
