Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 10-7007-F - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-MBD1 Antibody
- Antibody type
- Monoclonal
- Description
- MBD1 (Methyl-CpG-Binding Domain Protein 1) protein is a primary candidate for the readout of DNA methylation as it recruits chromatin remodelers, histone deacetylases and methylases to methylated DNA associated with gene repression. This protein, a member of a transcriptional repressor family MBD, is predominantly expressed in neurons. MBD protein binding requires both functional MBD domains and methyl-CpGs; however, some MBD proteins also bind unmethylated DNA and active regulatory regions via alternative regulatory domains or interaction with the NuRD/Mi-2 (Nucleosome Remodeling Deacetylase) complex members. The CXXC3 domain of MBD1 makes it a unique member of the MBD family due to its affinity to unmethylated DNA. MBD1 acts as an epigenetic regulator via different mechanisms, such as the formation of the MCAF1/MBD1/SETDB1 complex or the MBD1-HDAC3 complex. It also plays an important role in disease progression, contributes to the drug resistance of PC cells; however, the mechanism underlying the drug resistance endowed by MBD1 remains unknown.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antigen sequence
A partial length recombinant MBD1 p
rotein (amino acids 291-586) was us
ed as the immunogen for this antibo
dy.- Isotype
- IgG
- Antibody clone number
- ABM15H2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular FLOW staining of PMA treated Jurkat cells using 0.5 ug of antibody. Green represents FITC conjugated isotype control (Abeomics). Red represents FITC conjugated MBD1 (10-7007-F).
- Protocol
- Protocol