PA5-96402

antibody from Invitrogen Antibodies

Targeting: CLCN5 ClC-5, CLC5, DENTS, hCIC-K2, hClC-K2, NPHL1, NPHL2, XLRH, XRN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96402

Provider

Invitrogen Antibodies

Product name

CLC-5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: YPFLEAKEEF AHKTLAMDVM KPRRNDPLLT VLTQDSMTVE DVETIISETT YSGFPVVVSR ESQRLVGFVL RRDLIISIEN ARKKQDGVVS TSIIYFTEHS PPLPPYTPPT LKLRNILDLS PFTVTDLTPM EIVVDIFRKL GLRQCLVTHN GRLLGIITKK DVLKHIAQMA NQDPDSILFN; Positive Samples: SW480, U-937, HepG2, A673, NCI-H460, Mouse kidney, Mouse ovary, Mouse brain; Cellular Location: Cell membrane, Endosome membrane, Golgi apparatus membrane, Multi-pass membrane protein

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.33 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CLC-5 in extracts of various cell lines using CLC-5 Polyclonal Antibody (Product # PA5-96402) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of CLC-5 was performed in U2OS cells using CLC-5 Polyclonal Antibody (Product # PA5-96402).