Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B344 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B344, RRID:AB_2058336
- Product name
- IHC-plus™ AP1G1 / Adaptin Gamma 1 Antibody (aa625-675) LS-B344
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-AP1G1 Antibody - Western Blot. Western blot of Affinity Purified anti-AP1G1 antibody shows strong detection of a 91-kD band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.