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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB110-7877 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-7877, RRID:AB_2227424
- Product name
- Goat Polyclonal Gamma Adaptin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. AP1G1
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.7 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Gamma Adaptin Antibody [NB110-7877] - Analysis shows strong detection of a 91-kDa band corresponding to Human AP1G1 in a HeLa whole cell lysate (lane 1). Peptide competition (using 1 ug/ml of the immunizing peptide) blocks the specific reactivity of this antibody with AP1G1 (lane 2). Approximately 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP1G1 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature.system was used for signal detection (Roche) using a 60-sec exposure time.
